Protein Interactions Analysis in Infected Cells Using siRNA and WB

WB: β Actin Control Infected Control Infected Control Infected 1 1 NS1 siRNA M1 siRNA M2 siRNA 2 2 WB: NP WB: M2 WB: NS1 WB: M1 B A WB: β Actin WB: β Actin WB: β Actin 1 1 2 2 D C Control siRNA Control Infected NP siRNA Fig. S1

pEGFP-NS1 pEGFP-NP pEGFP pEGFP-M1 Control pEGFP-M2 Fig. S2

WB anti CLU IP anti NP IP anti M1 IP anti M2 IP anti NS1 WB NP WB NS1 PR8 IAV WB M2 WB M1 PR8 IAV CLU WB CLU 1 2 10% Input WB GAPDH 3 4 5 6 B A Fig. S3

Nascent Clusterin SP β chain α chain GST GST GST CLU Full length β chain α chain CLU β Chain β chain CLU α Chain α chain 6xHis IAV NP A Fig. S4

1 1 GST Pull down samples 1% input 1 2 3 4 1 2 3 4 GST pull down WB: His WB: His GST Pull down samples 1% input 2 2 1 2 3 4 1 2 3 4 C B GST pull down WB: GST WB: GST • CLU Full- GST + IAV NP-His • CLU β-GST + IAV NP-His • CLU α-GST + IAV NP-His • GST + IAV NP –His Fig. S4

1 2 3 Mock 0h 4h 8h 12h 4 WB: GAPDH WB: NP WB: CLU WB: Calnexin Fig. S5

NP Cyt C NP Cyt C Hoechst Merge Hoechst Merge pcDNA3.1 myc-NP pcDNA3.1 myc. A B Fig. S6

pCMV XL4- CLU pCMV Control 1 WB CLU Control Control siRNA 2 WB β Actin CLU siRNA A 1 WB CLU WB β Actin 2 B Fig. S7

Protein Interactions Analysis in Infected Cells Using siRNA and WB