Ch Muc4

1. B ChMuc4 Muc4 IIc/IIk CpMuc4 ChMuc4 Muc4 IIc/IIk CpMuc4 Fig A1. Polymorphic alleles of Muc4: (A) Phylogenetic tree of nucleotide sequences (from the 107th nucleotide to the end of the coding sequence) of all Bangladesh (Bang) and Indian (India) isolates from which Muc4 sequence was obtained. gp40/15 genotype is indicated after the isolate name, and the Genbank accession number follows. ChMuc4 Ia and CpMuc4 II are the published C. hominis and C. parvum sequences, respectively, and the CryptoDB (http://cryptodb.org/cryptodb/) gene name is give for these sequences. The tree was generated using Tree View (http://taxonomy.zoology.gla.ac.uk/rod/treeview.html) from alignments generated with ClustalW2 (http://www.ebi.ac.uk/Tools/clustalw2/index.html). Bar indicates 0.1 nucleotide substitutions per site. (B) Alignment of deduced amino acid sequences of the three Muc4 alleles, beginning at amino acid 17. Non-similar amino acids are in black type on gray, conservative substitutions are dark blue on light blue and identical sequences are red on yellow. A

2. B ChMuc5 Muc5 new CpMuc4 Muc5 IIc/IIk ChMuc5 Muc5 new CpMuc4 Muc5 IIc/IIk Fig A2. Polymorphic alleles of Muc5: (A) Phylogenetic tree of nucleotide sequences (from the 9th nucleotide to the end of the coding sequence) of all Bangladesh (Bang) and Indian (India) isolates from which sequence was obtained. Samples are labeled and the tree generated as described in Fig. A1A. (B) Alignment of deduced amino acid sequences of the four Muc5 alleles (complete coding sequence). Key is the same as Fig. A1B. A