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OXFAM-DELAGUA Water Analysis Kit Presentation

Learn about the components, capabilities, and maintenance of the OXFAM-DELAGUA water analysis kit. Details on bacteriological analysis process included.

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OXFAM-DELAGUA Water Analysis Kit Presentation

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Presentation Transcript

  1. OXFAM-DELAGUA Water analysis kit

  2. Título de la presentación Contents • Components • Capabilities • Maintenance • 4. Summary of bacteriological analysis process

  3. 1. Componentes 1. Components

  4. Filtration apparatus Incubator Culture Medium Petri Dishes Sampling Methods Turbidimeter pH and Chlorine meters Consumables

  5. Filtration apparatus

  6. Spares case

  7. External battery

  8. 2. Capabilities

  9. CAPABILITIES • Turbidity • pH • Free and total chlorine • Bacteriological analysis (thermotolerant faecal coliforms)

  10. 3. Maintenace

  11. GENERAL MAINTENANCE CAREFULLY READ THE MANUAL • Weekly • Wash, rinse and dry the filtration apparatus • Apply a smear of silicone grease to the black rubber O-ring • Charge the internal battery fully at the end of each week • Monthly • Check the incubator temperature and recalibrate if necessary • Check all the kit components

  12. BATTERY CAREFULLY READ THE MANUAL • Never allow the internal battery to discharge fully. • Never leave the incubator switched on for more than 18 consecutive hours • Always recharge the battery at the end of the working period in the field. • Recharge monthly during storage.

  13. 4. Summary of the bacteriological analysis process

  14. Preparation: Sterilization of equipment: (autoclave, pressure cooker, methanol, boiling) Petri: 15 min in autoclave or with methanol Filtration apparatus: 15 min with methanol Culture medium (38 gr/500 ml distilled water) Petri dishes (Up to 16 daily samples) Filtration process Filtration volume (10 ml, 50 ml,100 ml) Filtration control Incubation (16-18 H 44ºC) Counting colonies: Yellow colour (faecal coliforms)

  15. Presentation of results The results are presented as CFU/100ml CFU (Colony forming units) 100 ml of sample (treated or very clean water) have been filtered: CFU/100ml = nº of identified colonies as coliforms 50 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 2 10 ml of sample have been filtered: CFU/100ml = nº of identified colonies as coliforms x 10 It is possible to use smaller volumes in samples with higher contamination. The results should be calculated multiplying by the correspondent dilution factor.

  16. Thank you……….

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