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This document provides an in-depth overview of essential software for microarray analysis, especially useful for research and academic purposes. It covers key features like image analysis, data normalization, statistical analysis, and data visualization tools. Users can explore various software options from commercial, web-based, and freeware sources, including ArrayMaker Version 2, ScanAlyse, and GeneString 5.0. The guide aims to equip researchers with knowledge on tools that facilitate image loading, parameter selection, statistical analysis, and data clustering, enhancing their microarray data processing capabilities.
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Microarray Analysis Software Ricardo Verdugo ECS 289A – Winter 2003 University of California, Davis
Classes of software • Array experiment image analysis • Load image (.SCN , TIFF , others) • Select channels • Select parameter • Normalization • Gridding • Reading • Statistical analysis of raw data ???? • Discovery tools (you name it)
Some available software • Commercial Sources
Some available software • Web-based sources
Some available software • Freeware sources
Affymetrix Data [CEL] Version=3 [HEADER] Cols=640 Rows=640 TotalX=640 TotalY=640 OffsetX=0 OffsetY=0 GridCornerUL=227 235 GridCornerUR=4495 248 GridCornerLR=4462 4514 GridCornerLL=194 4500 Axis-invertX=0 AxisInvertY=0 swapXY=0 DatHeader=[0..46136] PoolA_Mg74AV2:CLS=4733 RWS=4733 XIN=3 YIN=3 VE=17 2.0 03/21/02 12:20:00 MG_U74Av2.1sq Algorithm=Percentile AlgorithmParameters=Percentile:75;CellMargin:2;OutlierHigh:1.500;OutlierLow:1.004 [INTENSITY] NumberCells=409600 CellHeader=X Y MEAN STDV NPIXELS 0 0 111.0 21.3 25 1 0 10116.5 1543.4 20 2 0 130.0 183.6 25 3 0 9843.0 1893.8 25 4 0 72.5 25.0 20 5 0 93.0 12.8 25 6 0 9556.0 1746.1 25 7 0 105.0 19.1 20 8 0 9213.0 2078.4 25 9 0 132.0 47.5 25 10 0 9685.3 1357.6 20 11 0 115.0 130.3 25 12 0 9346.0 1612.0 25 13 0 114.0 27.3 20 14 0 8730.0 1991.5 25
GeneString 5.0 • Data Normalization
GeneString 5.0 • Data Clustering
GeneString 5.0 • 3D Data Visualization
GeneString 5.0 • Pathway Views
GeneString 5.0 • Scripting