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Sperm Enzyme Preparation (Group 6)

Sperm Enzyme Preparation (Group 6). Comparing all Gradient Gel Standards. Experiment Objective. Compare all given gradient gel standards and use their relative mobilities to create the best fit mathematical model. Use best model to compare two gel samples. Gathering Data:. Gels 1 and 2.

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Sperm Enzyme Preparation (Group 6)

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  1. Sperm Enzyme Preparation (Group 6) Comparing all Gradient Gel Standards

  2. Experiment Objective • Compare all given gradient gel standards and use their relative mobilities to create the best fit mathematical model. • Use best model to compare two gel samples

  3. Gathering Data: Gels 1 and 2

  4. Hypothesis (Part One) • Standards will be reproducible.

  5. Hypothesis (Part Two) Has more proteins than

  6. Standards • Measured three times • Calculated relative mobilities • Calculated mean measurements • Best fit mathematical model

  7. Mathematical Models • Table of Molecular Weights and the Log of the Molecular Weights:

  8. Best Model Determination

  9. Model with its CI

  10. Comparison Chart

  11. The Reaction • The expansion of the Vitelline Envelope due to contact with sperm over a 30 minute time period.

  12. Comparing Unknowns to Standards • Vitelline Envelope control • Sperm enzyme control • Vitelline Envelope + sperm enzyme (t=0 min) • Vitelline Envelope + enzyme (t=5 min) • Vitelline Envelope + enzyme (t=10 min) • Vitelline Envelope + enzyme (t=15 min) • Vitelline Envelope + enzyme (t=30 min)

  13. Results: • Sperm enzyme controls between gels 1 and 2 were similar.

  14. Results: • Vitelline envelope controls are similar between gels one and two.

  15. Results: • In gel 1, the vitelline envelope remains the same throughout the experiment.

  16. Results: • Vitelline envelope breaks up as the bands become greater.

  17. Results: Standards

  18. Conclusion: • Standards are reproducible. • Sperm enzyme breaks up vitelline envelope. • Number of bands increase. • Small proteins are being detected.

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