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Early L(1)sc expression: Are there L(1)sc equivalence groups?

Early L(1)sc expression: Are there L(1)sc equivalence groups?. Stage 9 8 L(1)sc + cells. Early Stage 10 All posterior cells L(1)sc + 9-10 L(1)sc + cells + MP3&4 Total of 11 -12 L(1)sc + cells. Stage 10: 2 glia underneath MP3 lighter L(1)sc expression 7 lsc+ cells around MP4

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Early L(1)sc expression: Are there L(1)sc equivalence groups?

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  1. Early L(1)sc expression: Are there L(1)sc equivalence groups? Stage 9 8 L(1)sc+ cells Early Stage 10 All posterior cells L(1)sc+ 9-10 L(1)sc+ cells + MP3&4 Total of 11 -12 L(1)sc+ cells Stage 10: 2 glia underneath MP3 lighter L(1)sc expression 7 lsc+ cells around MP4 Total of 11 l(1)sc+ cells

  2. Lethal of Scute is Present in MPs and their Neuronal Progeny from Stages 9-11 L(1)Sc pdm2/nub merge Present in PMG (s10-12) Present in MNB Present in all MPs (MP4, MP3, MP1, MP5, MP6) and newly divided neurons (H-cell, H-cell sib; mVUM5,6 and iVUM5,6) Levels are asymmetric between H-cell and H-cell sib and between mVUM5 and iVUM5 l(1)sc may play a role in both neural precursor formation and establishing different daughter fates Stage 10 Stage 11 Stage 11 Stage 12

  3. Lethal of Scute is Present in MNB L(1)sc wor

  4. Reduced Numbers of Neurons are Present in AS-C Mutants Stage 12 Stage 14 Stage 16 Wild -type Df(1)scB57 6 elav+ cells present from late 11-16 Suggests that reduced numbers of neurons not due to cell death, but less neurons are generated

  5. AS-C Genes are Required for wor Expression wor merge Wild -type Df(1)scB57 wor: MNB Suggests MNB does not form and does not produce its 8 progeny

  6. CG8965 and wrapper are unaffected in AS-C mutants CG8965 wrapper scB57: 5 CG8965+ cells/seg 3.13 wrapper+ CG8965- cells/seg n= 8 seg High levels of wrapper did not always equal CG8965+ cells wt: 3 PMG/seg @ s14 n=2

  7. AS-C genes are Required for tbh Expression Tbh (mVUMs) Cas (mVUM4,5; iVUM4,5) Tkr (mVUM6; iVUM6) merge Wild -type Df(1)scB57 4 5 6 60% 50% mVUM iVUM Suggest that mVUM6 and iVUM6 are present 60% TbhCas Tkr

  8. AS-C Phenotype : Precursor Formation and Neuron-Specific Control AMG AMG AMG AMG PMG PMG PMG PMG Wild-type AMG AMG MP1 MP3 MP4 MP5 MP6 MNB Both L(1)sc and Notch MNBp H-cell sib Numb H-cell MP1 MP1 mVUM iVUM L(1)sc Notch Neither Notch nor AS-C

  9. AS-C Phenotype : Precursor Formation and Neuron-Specific Control AMG AMG AMG AMG PMG PMG PMG PMG AS-C mutant AMG AMG MP1 MP3 MP4 MP5 MP6 MNB Both L(1)sc and Notch X X H-cell sib Numb MP1 MP1 iVUM L(1)sc Notch Neither Notch nor AS-C

  10. 4 pdm2/nub+ Neurons are Present in AS-C mutants pdm2/nub merge Wild -type MP1 MP3 MP4 MP5 MP6 H-cell sib H-cell MP1 MP1 mVUM iVUM Df(1)scB57 Stage 14 pdm2/nub: MP1s, H-cell, H-cell sib

  11. AS-C Genes are Required for ple Expression ple (H-cell) CG13565 (H-cell sib) Lim3 (MP1s) Wild -type MP1 MP3 Df(1)scB57 H-cell sib MP1 MP1 H-cell pdm2/nub: MP1s, H-cell & H-cell sib present MP1 and H-cell sib gene expression unaffected AS-C genes required for H-cell specific gene expression

  12. AS-C mutants X X X X Df(1)scB57 D(f)sc19 X-ray derivative of In(1)ac3 pt mutation causes sc to lack C term Sc protein nonfunctional Rearrangement that separates all 5’ seq of ac ase ac X X X l(1)sc sc ac sc l(1)sc ase

  13. Scute is Present in MPs, but not Neurons from Stages 10-11 Sc pdm2/nub merge Stage 10 Present in PMG (s10-11) Present in MP3, MP5, MP1, MP6, and MNB prior to division and is absent from newly divided neurons May play a role in neural precursor formation, but not in establishing different daughter fates Stage 11 Stage 11 Stage 11

  14. Achaete Expression Ac pdm2/nub merge Stage 10 Present in MP1 and transiently in MP5, MP6, and MNB Remains on in MP1 neurons to end of embryogenesis May play a role in precursor formation and in MP1 neuron development Stage 11 Stage 11 Stage 12

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