Lab Demonstration of Enteric Bacteria - PowerPoint PPT Presentation

dr amel yacoubi n.
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Lab Demonstration of Enteric Bacteria

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  1. Dr. Amel Yacoubi LabDemonstration of EntericBacteria

  2. On Gram staining: Gram positive cocci in groups (Figure n°1) On blood agar: beta hemolysis (complete hemolysis) (Figure n°2) On Mannitol salt agar : fermentation of mannitol (yellow color) (Figure n°3) Coagulase test: positive (Figure n°4) Organism: Staphylococcus aureus Station 1

  3. Staphylococcus aureus (Figure n°1) (Figure n°2) (Figure n°4) (Figure n°3)

  4. Microscopic : Gram negative rods (Figure n°5) • Culture on Mac conkey agar: • Lactose fermenting (LF) colonies (pink colonies) (Figure n°6) • Ex: E.coli • Non Lactose fermenting (NLF) (Figure n°7 ) • Ex: Salmonella – Shigella Station 2:

  5. Salmonella – shigella/ E.coli (Figure n°6) (Figure n°5) (Figure n°7)

  6. TCBS agar • Use: selective medium of Vibriocholorae (Figure n°8 ). • Growth: V. cholorae gives yellow colonies due to fermentation of sucrose. Others vibrios give pale colonies because they not ferment sucrose (Figure n°9 ) . Station 3:

  7. Vibriocholorae (Figure n°8) (Figure n°9)

  8. Station 4: •  Gram positive bacilli showing endospores (Figure n°10) • Ex: Bacillus anthracis Bacillus anthracis (Figure n°10)

  9. Gram positive rods with variable staining (Figure n°11 ) • Ex: Clostridium perfringens • Culture on Robertson cooked meat: Anaerobic medium used for isolation of Cl. Perfringens and others anaerobic bacteria. • The color turned orange due to the saccharolytic activity of organism (Figure n°12). Station 5:

  10. Clostridium perfringens (Figure n°11) (Figure n°12)

  11. Station 6: • Anaerobic jar (Figure n°13) Anaerobic culture Anaerobic Gas Pack (Figure n°13)