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A. B. CO 2. Carbon source. Ratio in headspace. Growth (cells per ml ). NADPH. NADP-dependent formate dehydrogenase Dhaf_4268-71. CO:N 2. 40:60. < 0.1 x 10 3. 2e -. NADP +. CO 2 :N 2. 40:60. < 0.1 x 10 3. formate. THF + ATP.
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A. B. CO2 Carbon source Ratio in headspace Growth (cells per ml) NADPH NADP-dependent formate dehydrogenase Dhaf_4268-71 CO:N2 40:60 < 0.1 x 103 2e- NADP+ CO2:N2 40:60 < 0.1 x 103 formate THF + ATP CO:CO2:N2 30:30:40 64 x 103 formyltetrahydrofolate synthetase Dhaf_0555, Dhaf_1049 ADP H2:CO:N2 30:30:40 66 x 103 H2:CO2:N2 30:30:40 44 x 103 formyl-THF H+ methenyltetrahydrofolate cyclohydrolase Dhaf_0557, Dhaf_3496 H2O methenyl-THF 2e- methylenetetrahydrofolate dehydrogenase Dhaf_0557, Dhaf_3496 NADPH NADP+ 2e- methylene-THF acetyl-CoA CO 2e- methylenetetrahydrofolate reductase Dhaf_0077 Vanillate + THF DHB methyl-THF CO2 O-demethylase 20 homologs in DCB-2 H+ CFeSP methyltransferaseDhaf_2792 carbon monoxide dehydrogenase Dhaf_0882, Dhaf_1166 Dhaf_2798, Dhaf_3792 H2O THF CO methylated CFeSP Carbonyl branch Methyl branch CoA acetyl-CoA synthaseDhaf_2797 CFeSP acetyl-CoA