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pGLO Transformation Lab

pGLO Transformation Lab. Many organisms can glow!. Jellyfish. Amphipods Oceanexplorer.noaas.gov/explorations/05deescope/background/fluorescence. www.worldnetcams.com/sealife/cerianthus.jpg. Corals. Copepods Oceanexplorer.noaas.gov/explorations/05deescope/background/fluorescence.

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pGLO Transformation Lab

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  1. pGLO Transformation Lab

  2. Many organisms can glow! Jellyfish Amphipods Oceanexplorer.noaas.gov/explorations/05deescope/background/fluorescence www.worldnetcams.com/sealife/cerianthus.jpg Corals Copepods Oceanexplorer.noaas.gov/explorations/05deescope/background/fluorescence www.afae.it/pages/galleria%20immagini/Invertebrati%20marini/trachy.htm www.wileywiggins.com/ 2003_05_01_blog2.html www.welc.cam.ac.uk/ ~pineslab/Projects/gfp.htm Renilla reniformis

  3. Crystal Jelly has a gene called GFP that makes it glow! when something glows, it is fluorescent

  4. Transgenic Mice Transgenic = something that has genes from different organisms (individuals) To remember... trans = move genic = about genes So transgenic = genes moved from one organism to another

  5. E. coli = bacteria bacteria = very small organisms that have DNA but no nucleus They were some of the first life on earth! In this lab, we will make bacteriaglow!!

  6. DNA RNA Protein Trait Green Fluorescent Protein • GFP Gene • found in jellyfish • put into bacteria GLOWING CELLS Why should we do this? TO DO BIOTECHNOLOGY!

  7. Bacterial chromosome How will we do this? Transformation. Plasmid with recombinant DNA! Bacterial chromosome Transformation is when the DNA from one organism is combined with the DNA from another organism. Bacteria now make protein that glows…

  8. STARTING MATERIALS E. coli cells • sensitive to antibiotics • can’t glow • can be transformed Bacterial chromosome

  9. GROW ON AN AGAR PLATE AmpR GFP Ara … that can GLOW! END RESULT Bacteria with Recombinant DNA…

  10. Makes the bacteria glow Helps the bacteria make GFP when it eats the sugar arabinose makes all transformed bacteria resistant to ampicillin Genes on the Plasmid AmpR pGLO plasmid GFP Ara

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