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Plasmids

Plasmids. Tool #3: Plasmids. Job=Copy DNA Small, self-replicating circular DNA In bacteria Can insert a gene into plasmid. What can you do with Plasmids?. Transformation: Insert recombinant plasmid into bacteria Culture recombinant bacteria = make clones

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Plasmids

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  1. Plasmids

  2. Tool #3: Plasmids • Job=Copy DNA • Small, self-replicating circular DNA • In bacteria • Can insert a gene into plasmid

  3. What can you do with Plasmids? • Transformation: • Insert recombinant plasmid into bacteria • Culture recombinant bacteria = make clones • Allows you to get multiple copies of a specific gene or DNA sequence

  4. Recombinant Plasmid • Antibiotic resistant genes as a marker • Restriction sites for splicing in gene of interest

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