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ABSTRACT

ABSTRACT. Hepatoma Cancer Modelling: Research of the Preventive and Curative Effect of Andrographis paniculata Ness. in the Rat Induced by DMBA. The present study was designed to investigate histological alteration of hepatosit

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ABSTRACT

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  1. ABSTRACT Hepatoma Cancer Modelling: Research of the Preventive and Curative Effect of Andrographis paniculata Ness. in the Rat Induced by DMBA The present study was designed to investigate histological alteration of hepatosit influenced by DMBA in the Sprague-Dawley rat. DMBA-induced hepatoma is a useful model to investigate the changes of hepatosit cells that occur during hepatoma progression. Hepatoma model was induced 10 times twice a week by oral DMBA 20 mg/kg body weight. Hepatoma occurred in 45 % animals nine weeks after oral administration of DMBA, it was represented with texture and nodule on the liver and the increasing of liver volume compare with normal control (F(1,12) = 7,343; p<0,05). This study was also designed to investigate the preventive and curative effect of Andrographis paniculata Ness. extract on hepatoma induced by DMBA. Preventive administration of three different dose of Andrographis paniculata Ness. (100 mg/kg, 300 mg/kg and 1000 mg/kg) were all maintain liver volume as good as the normal control and significantly different with liver volume of DMBA treated rat (F(4,37) = 11,070, p<0,001). Andrographis paniculata Ness. has the preventive effect on limiting the frequency of cancer initiation and growth. However, curative administration of three different dose of Andrographis paniculata Ness. (100 mg/kg, 300 mg/kg and 1000 mg/kg) were showed liver volume as good as the normal control and significantly different with liver volume of DMBA treated rat (F(5,39) = 8,219, p<0,001). So, Andrographis paniculata has significant effect on hepatoma modelling rat induced by DMBA. The Epithelial cell were harvested on day 90 and stained on routine histology staining, hematoxylineosin, for morphological qualitative analysis. The lesions observed from the removed samples ranged widely from benign to malignant. The results showed that DMBA induce cell proliferation, nuclear irregularities and induced cell necrosis. Preventive and curative effect of Andrographis paniculata Ness. inhibit cell proliferation. Finally, we hope that the research will provide some clues towards early detection and, hopefully, prevention and treatment of hepatoma. Keyword(s) : DMBA, hepatoma, HCC, Andrographis paniculata Ness., Sprague Dawley.

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