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Enabling marker-assisted breeding in Rosaceae

RosBREED. RosBREED. Enabling marker-assisted breeding in Rosaceae. PROJECT OVERVIEW. Outline. The RosBREED Project The Need for RosBREED Major Activities RosBREED is Underway. RosBREED. The RosBREED Project. We’re In It Together. CROPS of the ROSACEAE FAMILY. RosBREED.

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Enabling marker-assisted breeding in Rosaceae

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  1. RosBREED RosBREED Enabling marker-assisted breeding in Rosaceae PROJECT OVERVIEW

  2. Outline • The RosBREED Project • The Need for RosBREED • Major Activities • RosBREED is Underway RosBREED

  3. The RosBREED Project

  4. We’re In It Together CROPS of the ROSACEAE FAMILY RosBREED

  5. The RosBREED Project • Vision Integrationof modern genomics tools with traditional breeding approaches will transform crop improvement in Rosaceae, significantly improving profitability and sustainability of U.S. rosaceous crop industries and contributing to increased consumption and enjoyment of these fruit, nut, and floral products. RosBREED

  6. The RosBREED Project • Mission We will create a dynamic, sustained program in research, infrastructure establishment, training, and outreachfor developing and applying marker-assisted breeding based on improved knowledge of industry value and consumer preferences to accelerate and increase the efficiency of rosaceous cultivar release and successful cultivaradoption. RosBREED

  7. The RosBREED Approach Socio-economics information Objectively define SELECTION TARGETS IMPROVED CULTIVARS Optimize SELECTION STRATEGIES DNA information RosBREED

  8. Project Objectives • Enhance new cultivar adoption, enlarge market potential, increase consumption • – socio-economics knowledge of trait values 2) Establish sustainable infrastructure for MAB in Rosaceae – MAB Pipeline components 3) Integrate breeding and genomics information into breeding – breeding information management system 4) Conduct MAB in demo breeding programs, fruit quality – MAB Pipeline implementation 5) Enhance sustainability of cultivar development – MAB tech transfer to breeders, engage other stakeholders RosBREED

  9. The RosBREED Project • PI: Amy Iezzoni, Michigan State University • 4 years, Sep 2009 – Aug 2013 • Funded by SCRI (USDA-NIFA’s Specialty Crop Research Initiative) • $14.4 M ($7.2 M SCRI, $7.2 in-kind Partners) RosBREED

  10. The RosBREED Project • Centered on breeders & breeding programs • U.S.-wide and international collaboration • 10 Teams (Socio-Economics, Industry, Breeding, Pedigree-Based Analysis, Breeding Information Management System, Genomics, Genetics, Marker-Assisted Breeding Pipeline, Extension, Administration) • 32 members of 3 Advisory Panels (Stakeholder, Extension, Scientific) RosBREED

  11. Breeding Program Locations RosBREED

  12. RosBREED’s Demonstration Breeders NnadozieOraguzie Washington State Univ. Amy Iezzoni Michigan State Univ. RosBREED

  13. RosBREED’s Demonstration Breeders Jim Luby Univ. Minnesota Kate Evans Washington State Univ. Susan Brown Cornell Univ. RosBREED

  14. RosBREED’s Demonstration Breeders Chad Finn Oregon State Univ. Jim Hancock, strawberry breeder Tom Davis Univ. New Hampshire RosBREED

  15. RosBREED’s Demonstration Breeders David Byrne Texas A&M KsenijaGasic Clemson Univ. Tom Gradziel UC Davis John Clark Univ. Arkansas RosBREED

  16. International Partners Plant Research Intl., The Netherlands East Malling Research, United Kingdom INRA – Bordeaux, Avignon & Angers, France CRA-FRU Rome, Italy Andres Bello University, Chile ARC, South Africa Plant & Food Research, New Zealand RosBREED participant location

  17. Distribution & Processing Consumer & Markets Systems Approach, Three Advisory Panels Production Robert Curtis Henry Bierlink Ron Perry Clark Seavert Brian Sparks Fred Bliss Fred Cook Rick Harrison Chrislyn Particka Gary van Sickle Chris Watkins Lailing Cheng Phil Simon Jim Allen Chalmers Carr III Bill Dodd Bruce Grim Philip Korson Jessica Goldberger Mercy Olmstead Jamie Sherman PereArús Joe Avari Peter Hirst Bert Abbott Robin Buell Susan Gardiner Tom Stokes Phil Baugher Advisory Panel Color Key Industry Extension Scientific David Karp Carolyn Ross Kevin Moffitt RosBREED

  18. The Need for RosBREED

  19. skeptical dismissal good intentions The Chasm Breeding Genomics

  20. Bridging the Chasm RosBREED RosBREED Breeding Genomics

  21. Decision support DNA information Bridging the Chasm RosBREED RosBREED SE info Breeding Genomics Socio-economics Programs Research Research More efficient development of new cultivars Genomics Resources

  22. Genomics Breeding Research Programs Consumer & Industry Values Socio-economics information DNA information The MAB Pipeline Routine Breeding Operations

  23. Overview of Activities TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING GERMPLASM CHOICE DNA information PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit! MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  24. The Goal: Routine MAB TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING DNA information GERMPLASM CHOICE PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  25. Major Activities

  26. Socio-economics TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING GERMPLASM CHOICE DNA information PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  27. Germplasm & Phenotyping TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING GERMPLASM CHOICE DNA information PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  28. Genome Scans TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING GERMPLASM CHOICE DNA information PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  29. QTL Discovery TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING DNA information GERMPLASM CHOICE PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  30. Fast-tracked Pipelining TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Promising targets Breeding markers Screening services MAB PIPELINING GERMPLASM CHOICE DNA information PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  31. Breeders’ Toolbox TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING GERMPLASM CHOICE DNA information PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  32. Technology Transfer TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING DNA information GERMPLASM CHOICE PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  33. News & Events TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Socio-economics information GENOME SCANS Marker priorities Screening services Breeding markers MAB PIPELINING GERMPLASM CHOICE DNA information PBA: crop-wide STANDARDIZED PHENOTYPING New QTLs discovered PBA: breeding program Crossing scheme Efficient routine breeding operations Better fruit MAB PIPELINING Seedling selection scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT

  34. RosBREED is Underway

  35. Meetings RosBREED I First Annual Meeting, 7-8 Jan 2010 RosBREED II Annual Advisory Panel Meeting, 13 Jan 2011

  36. Website: www.rosbreed.org Various information & resources

  37. Acknowledgements This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

  38. Cornell Susan Brown KenongXu Clemson KsenijaGasic Gregory Reighard Texas A&M Dave Byrne Univ. of CA-Davis Tom Gradziel Carlos Crisosto Univ. of New Hamp. Tom Davis MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang CholaniWeebadde WSU Cameron Peace Dorrie Main Kate Evans Karina Gallardo Raymond Jussaume Vicki McCracken Nnadozie Oraguzie Mykel Taylor Univ. of Arkansas John Clark USDA-ARS Nahla Bassil Gennaro Fazio Chad Finn Univ. of Minnesota Jim Luby Chengyan Yue Oregon State Univ. Alexandra Stone Plant Research Intl, Netherlands Eric van de Weg Marco Bink

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