OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION

1. OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals

2. Goal What • Integrate modern genomics tools with traditional breeding approaches, systematically (8-stage MAB Pipeline) Impact • Enrich breeding material for favorable geneticsto efficiently increase likelihood and accuracyof identifying top performers RosBREED

3. Year 2 Deliverables What • Two Jewels (fast-tracked genetic tests) in routine use To whom • WSU apple and sweet cherry breeding programs Impact • est. $80Ksavedfrom2011cherryseedlingcullingalone! RosBREED

4. Year 2 Deliverables What • CommunityBreedersPage-regulararticleinNewsletter MAB Pipeline Nov‘10 apple Jewel use Feb‘11 Pipeline in action May‘11 peach Jewel use Aug‘11 To whom cherry Jewel use Nov’11 • Community Breeders Impact • Not evaluated – but represents early start on Yr 4 tech transfer RosBREED

5. Challenges and Plan for tackling • Moving breeding programs forward together, keeping all on same page • Requires success stories but also consistent& constant advice • ...need more people on Team • Some enacted solutions: • Community Breeders Page • coordinated master datafiles • much involved in other Team activities RosBREED

6. Planned Year 3 Deliverables What • Routine “MAB Pipelining” by Demonstration Breeders • First: fast-tracked markers into routine use • Soon after: New QTLs from PBA with genome scans To whom • Demonstration Breeders (Yr 3)  other breeders (Yr 4) Intended Impact • Success stories & experiences useful for Yr 4 “tech transfer” to all U.S. Rosaceae breeders. RosBREED

7. OBJ. 5 EXTENSION Year 2 Deliverables, Challenges, Year 3 goals

8. Goals • Breeders – adoption of Marker-Assisted Breeding • Allied Scientists - utilization of newly generated science based knowledge, tools and databases • Industry – better understanding of the power of DNA-informed breeding leads to new ways to jointly accelerate commercialization and impact of new cultivars RosBREED

9. Year 2 Deliverables 78/130 recipients opened the Nov 2011 newsletter and Community breeders said: What • Project-wide content – developed, disseminated and undertaken “RosBREED newsletter stands out among other similar project newsletters” To whom • Project participants & stakeholders “it builds a sense of community” “circulate to a wider audience” Impact • Stakeholders gain knowledge of project activities and deliverables. Stats: in 2011 the RosBREED website was visited by 2,242 unique visitors, from 79 countries. Since creation, 5,541 visits from US - ALL 50 states

10. Year 2 Deliverables What • Expansion of RosBREED network through presentations at meetings and informal gatherings – conducted ASHS 2011 poster and oral presentations To whom • Breeders, allied scientists, industry stakeholders Impact • Awareness and interest created at national and international meetings including “unveiling” of Jewels

11. Obj 5: Year 2 Deliverables What • Assist in surveys - Socio‐Economics Team grower mail‐in surveys and clicker surveys at grower meetings To whom • Market intermediaries and growers Impact • Growers help identify valuable Jewels and are more knowledgeable of their local breeding programs

12. Challenges and Plan for Tackling • Video content obtained, free computer software programs for editing existing videos is limited • Experiment with softwares that have been used successfully in other extension venues • Obtain participation of a high percentage of Rosaceae Community Breeders at the “MAB in Action” Workshop • Hold Workshop the day prior to the ASHS meeting in Miami; generate interest in the Workshop through publicizing success stories Computer Demo: RosBREED Videos

13. Planned Year 3 Deliverables What • RosBREED Participatory Workshop: “Marker-Assisted Breeding in Action”, 30 July 2012 – promote, prepare & conduct • Visit breeding programs on request to share RosBREED’sdeliverables To whom • Demonstration and Community breeders Intended Impact • Comprehension by breeders of available knowledge, technologies and software for conducting routine MAB; application of MAB in their breeding programs

14. Planned Year 3 Deliverables What • Disseminate RosBREED deliverables and communicate with stakeholders through the use of published materials and participation in meetings To whom • Rosaceae breeders, allied scientists, industry Intended Impact • Breeders: breed as efficiently as possible • Allied scientists: use newly generated science-based knowledge, tools, databases • Industry:– interact effectively with local breeding programs, understand power of DNA-informed breeding