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Proteomic characterization of the human centrosome by protein correlation profiling

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Proteomic characterization of the human centrosome by protein correlation profiling

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    1. Proteomic characterization of the human centrosome by protein correlation profiling Andersen et al 2003 Nature

    3. Main point of the paper Validated 23 (4 by other groups) novel components and by in vivo localiztion (EGFP-tagging); and identified 41 candidates of the centrosome with the Protein Correlation Profiling (PCP) algorithm.

    4. Sample preparation and separation

    10. The differential subcellular localization of closely related protein isoforms can be distinguished by this approach. 14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. 14-3-3 proteins have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. There are seven genes that encode 14-3-3s in most mammals and 13-15 genes in many higher plants. 6-phosphofructokinase, type C; D-3-phosphoglycerate dehydrogenase; Peptidyl-prolyl cis-trans isomerase A; identified in the peak fraction but are not controsomal. The differential subcellular localization of closely related protein isoforms can be distinguished by this approach. 14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. 14-3-3 proteins have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. There are seven genes that encode 14-3-3s in most mammals and 13-15 genes in many higher plants. 6-phosphofructokinase, type C; D-3-phosphoglycerate dehydrogenase; Peptidyl-prolyl cis-trans isomerase A; identified in the peak fraction but are not controsomal.

    11. A Chaotropic agent is an agent which causes molecular structure to be disrupted; in particular, those formed by nonbonding forces such as hydrogen bonding, Van der Waals interactions, and the hydrophobic effect. A Chaotropic agent is an agent which causes molecular structure to be disrupted; in particular, those formed by nonbonding forces such as hydrogen bonding, Van der Waals interactions, and the hydrophobic effect.

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