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Microscopy

Microscopy. micrometers. Microscopes help us to see small objects/specimens. These objects are typically less than 500 micrometers – the size of a human egg. We, typically, cannot see objects smaller with the human eye. The size of a pencil point.

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Microscopy

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  1. Microscopy

  2. micrometers Microscopes help us to see small objects/specimens. These objects are typically less than 500 micrometers – the size of a human egg. We, typically, cannot see objects smaller with the human eye. The size of a pencil point. 1 micrometer = 1/1000 mm

  3. resolution The ability to distinguish the details of an image.

  4. resolution We improve the resolution of an image by adjusting the focus of a microscope. The resolution capability of a microscope of may be determined by an object’s size and degree of detail.

  5. magnification The process of enlarging an object’s appearance – not the object itself.

  6. magnification We indicate the magnification of an object by _____ X. For example an object that is 100X had been magnified 100 times. The bottom image is 1860X.

  7. Magnification Objects are magnified by microscope. Different microscopes have different magnification capabilities.

  8. Compound light microscope Compound means more than one lens working together to magnify an image. Light refers to the illumination of the object to increase clarity.

  9. Compound light microscope The magnification of an object observed using this microscope is the multiplication of the 2(+) lenses used to view the object. What is the possible compounded magnification of these lenses?

  10. Compound light microscope

  11. Compound light microscope The maximum magnification for a compound light microscope is about 1000X. These are bacteria cells that are each less than 8 micrometers in size.

  12. Robert Hooke A dead British guy by the name of Robert Hooke was the first to look at plant cells using a compound light microscope. He thought that the cork cells looked like ‘little rooms’ and, therefore, called them cellulae (cell in Latin).

  13. Anton von Leuwenhoek A Danish guy by the name of Anton von Leuwenhoek was the first to look at protozoa with a compound light microscope. He called these animal like cells a ‘animalcules’.

  14. Cell theory As a result of new technology – new of the 1600’s – the world of life opened to scientists. This allow scientists to conclude that 1) all life is made up of cells, 2) all cells come from preexisting cells, and 3) cells are the smallest unit of life.

  15. Electron microscope Electron microscopes shoot electrons at dead objects/specimens. As the electrons bounce back, the energy is recorded and translated into an image.

  16. Scanning electron microscope Scanning electron microscopes create a 3D image of an object.

  17. Scanning electron microscope The maximum magnification of scanning electron microscopes is approximately 500,000 times.

  18. Transmission electron microscope Transmission electron microscopes create images that are 2D and only of nonliving objects/specimens. The maximum magnification is about 1,000,000X.

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