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Microscopes

Microscopes. Drawing Rules, Magnification & Field of Vie w. Microscope Drawing Rules. Use a pencil Neat, ruled lines that do not overlap All labels to the right No arrows on lines Each diagram = at least half a page. Microscope Drawing Rules. Stipple in darker areas (no shading)

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Microscopes

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  1. Microscopes Drawing Rules, Magnification & Field of View

  2. Microscope Drawing Rules • Use a pencil • Neat, ruled lines that do not overlap • All labels to the right • No arrows on lines • Each diagram = at least half a page

  3. Microscope Drawing Rules • Stipple in darker areas (no shading) • Magnification should be written at the bottom of the diagram • Descriptive Title Should be UNDERNEATH the drawing (Figure 1: ___________)

  4. Field of View (FOV) Field of View – the viewyou actually see when you look through a microscope

  5. Microscope Calculations

  6. Field of View (FOV) Field of View – the viewyou actually see when you look through a microscope

  7. Low Field of View (Low FOV) Low FOV = measured with a ruler on the microscope • Count the spaces you see • Count the halves as well • Here we see 5.5 spaces • So Low FOV = 5.5 mm

  8. Medium & High Field of View (FOV) Medium FOV = low FOV X _MagnificationLOW_ MagnificationMEDIUM High FOV = low FOV X _MagnificationLOW_ MagnificationHIGH REMEMBER…we only use a ruler for LOW FOV

  9. Let’s Practice If the low FOV = 2mm, low magnification is 4x, medium magnification is 10x and high magnification is 40x, calculate the high FOV.

  10. Let’s Practice If the low FOV = 2mm, low magnification is 4x, medium magnification is 10x and high magnification is 40x, calculate the high FOV. High FOV = 2mm x _____4x___ 40x = 2 mm x 0.1 = 0.2 mm or 200µm

  11. Let’s Practice If the low FOV = 2mm, low magnification is 4x, medium magnification is 10x and high magnification is 40x, calculate the high FOV. High FOV = 2mm x _____4x___ 40x = 2 mm x 0.1 = 0.2 mm or 200µm

  12. Specimen Size You can estimate specimen size using the field of view. For example, if your field of view is 200 µm, and the specimen takes up about a quarter of the space, you can estimate it to be about 50 µm.

  13. Drawing Magnification/Scale • When you are asked to draw what you see under the microscope, your drawings will be much larger than your specimen. You need to indicate approximately how much larger than life your drawings are. Magnification = drawing size or M = D Actual size A

  14. Drawing Magnification/Scale • e.g. a student, observing an amoeba under a magnification of 400X, calculates that it is about 50m long. If she then draws the amoeba 8 cm long, what is the magnification and scale of her drawing? Magnification = drawing size = 80000 µm Actual size 50 µm = 1600 X, and the scale is 1cm = 6.25 µm.

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