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quantitative amino acid analysis
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Presentation Transcript
Hydrolysis is typically achieved under acid conditions. A standard procedure is hydrolysis with 6 M hydrochloric acid (24 hours, 110°C). Fragile amino acids, especially tryptophan and cysteine, will be partially destroyed. And then hydrolyzed samples (amino acids) are derivatized pre-column or post column for sensitive detection, separated by RP/SCX column. The use of internal and external standards of known amount is crucial for accurate quantification of each amino acid.
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