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  1. Version: 1 Revision Date: 11/30/2012 PerCP/Cy5.5 anti-mouse CD25 Antibody Catalog# / Size 101912 / 100 µg 101911 / 25 µg 3C7 (See other available formats) Clone Other Names IL-2Rα, Ly-43, p55, Tac Isotype Rat IgG2b, κ 0 reviews Ave. Rating ★ ★ ★ ★ ★ Description CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre -B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor. Reactivity Mouse Con A-stimulated C57BL/7 splenocytes (Day 3) stained with 3C7 PerCP/Cy5.5 IL-2-dependent BALB/c mouse helper T-cell clone HT-2 Immunogen Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Formulation The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody. Preparation 0.2 mg/ml Concentration The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Storage & Handling FC - Quality tested Application Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. Recommended Usage Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors , and immunohistochemical staining of acetone- fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The LEAF™Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101906). Application Notes 1,2 1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block) 2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block) 3. Lei F, et al. 2013. J Vis Exp. 60:3986. PubMed. Application References (PubMed link indicates BioLegend citation) Publication Library Antigen Details Structure Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD Activated T and B cells, thymocyte subset, pre-B cells, T regulatory cells Distribution IL-2 receptor, signal transduction Function IL-2 Ligand Receptor 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. Antigen References 16184 Gene ID

  2. View information about CD25 on UniProt.org UniProt Documentation Technical data sheet Certificate of Analysis Safety Data Sheet Related Protocols Cell Surface Immunofluorescence Staining Protocol Related FAQs How stable is PerCP/Cy5.5 tandem as compared to PerCP alone? PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation. Related Categories Immunology > Mouse Immunology > CD and Related Molecules > CD25 > 3C7 > Immunology > Mouse Immunology > Cytokine and Chemokine Receptors > CD25 (IL-2 Rα) > 3C7 > Immunology > Mouse Immunology > T Regulatory Cells (TReg) > CD25 (IL-2Rα) > Immunology > Mouse Immunology > Stem Cells > CD25 (IL-2Rα) > Immunology > Mouse Immunology > B Cells > CD25 > Cytokines & Chemokines > Mouse Cytokine & Chemokine Research > Antibodies to Receptors > IL-2 Rα (CD25) > 3C7 > Immunology > Mouse Immunology > Costimulatory Molecules > CD25 > 3C7 > Other Formats Description Clone Applications LEAF™ Purified anti-mouse CD25 3C7 Block, FC, IHC, CyTOF® PE anti-mouse CD25 3C7 FC Purified anti-mouse CD25 3C7 FC, IHC, CyTOF® FITC anti-mouse CD25 3C7 FC APC anti-mouse CD25 3C7 FC Purified anti-mouse CD25 (MaxPar® Ready) 3C7 FC, CyTOF® PE/Cy7 anti-mouse CD25 3C7 FC APC/Cy7 anti-mouse CD25 3C7 FC

  3. For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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