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THE LIGHT MICROSCOPE AND THE ELECTRON MICROSCOPE

THE LIGHT MICROSCOPE AND THE ELECTRON MICROSCOPE. © 2007 Paul Billiet ODWS. UNITS OF MEASUREMENT. 1m = 10 3 mm (millimetres) 1m = 10 6 µm (micrometres) 1m = 10 9 nm (nanometres) Sometimes in old texts Angstroms (Å) are used (the diameter of a hydrogen atom) 1m = 10 10 Å .

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THE LIGHT MICROSCOPE AND THE ELECTRON MICROSCOPE

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  1. THE LIGHT MICROSCOPE AND THE ELECTRON MICROSCOPE © 2007 Paul Billiet ODWS

  2. UNITS OF MEASUREMENT 1m = 103 mm (millimetres) 1m = 106 µm (micrometres) 1m = 109 nm (nanometres) Sometimes in old texts Angstroms (Å) are used (the diameter of a hydrogen atom) 1m = 1010 Å © 2007 Paul Billiet ODWS

  3. The electromagnetic spectrum © 2007 Paul Billiet ODWS

  4. Light v Electron © 2007 Paul Billiet ODWS

  5. Magnifying using the Light Microscope © 2007 Paul Billiet ODWS

  6. Magnifying using the Electron Microscope © 2007 Paul Billiet ODWS

  7. FEATURE LIGHT MICROSCOPE ELECTRON MICROSCOPE Electromagnetic spectrum used Visible light 760nm (red) – 390nm Colours visible Electrons app. 4nm Monochrome Maximum resolving power app. 200nm 0.2nm Fine detail Maximum magnification x1000 – x1500 x500 000 Radiation source Tungsten or quartz halogen lamp High voltage (50kV) tungsten lamp Lenses Glass Magnets Interior Air-filled Vacuum Focussing screen Human eye (retina), photographic film fluorescent (TV) screen, photographic film THE LIGHT MICROSCOPE v THE ELECTRON MICROSCOPE © 2007 Paul Billiet ODWS

  8. FEATURE LIGHT MICROSCOPE ELECTRON MICROSCOPE Preparation of specimens Temporary mounts living or dead Tissues must be dehydrated = dead Fixation Alcohol OsO4 or KMnO4 Embedding Wax Resin Sectioning Hand or microtome slices  20 000nm Whole cells visible Microtome only. Slices  50nm Parts of cells visible Stains Water soluble dyes Heavy metals Support Glass slide Copper grid THE LIGHT MICROSCOPE v THE ELECTRON MICROSCOPE © 2007 Paul Billiet ODWS

  9. Embedding in resin © 2007 Paul Billiet ODWS

  10. Microtome knife © 2007 Paul Billiet ODWS

  11. Copper grid slides © 2007 Paul Billiet ODWS

  12. © 2007 Paul Billiet ODWS CELL STRUCTURE AND MICROSCOPY

  13. CELL STRUCTURE AND MICROSCOPY © 2007 Paul Billiet ODWS

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