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MS/MS Spectrum Interpretation Exercises for Peptide Mass Identification

Practice manual MS/MS spectrum interpretation for peptide mass and sequence identification. Utilize provided tools for sequence search and modification identification.

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MS/MS Spectrum Interpretation Exercises for Peptide Mass Identification

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  1. Name: Matrikelnumber: Exercise 1. MS/MS manual spectrum interpretation In the raw file go to scan number: 11169 Move to the corresponding MS1 scan to determine the charge state and peptide mass Solve the MS/MS spectrum Use the peptide mass and sequence for a Sequence search using: http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=SQ Search format: Mpepseq(pep. Sequence) Example: 1222.66 seq(LIEDNEYTAR)

  2. 20130226_1ugA431 # 11169 RT: 52.11 AV: 1 NL: 2.14E4 T: FTMS + c NSI d Full ms2 757.90@hcd30.00 [100.00-1530.00] 1182.63 100 95 90 85 80 75 70 591.82 65 60 55 Relative Abundance 50 45 1085.58 40 857.47 35 201.12 213.09 30 970.56 25 20 742.44 244.16 15 657.34 441.20 314.15 1400.70 10 515.32 5 1313.63 939.48 781.40 1450.98 0 200 400 600 800 1000 1200 1400 m/z

  3. Exercise 2. MS/MS manual spectrum interpretation In the raw file go to scan number: 3692 Move to the corresponding MS1 scan to determine the charge state and peptide mass Solve the MS/MS spectrum Use the peptide mass and sequence for a Sequence search using: http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=SQ Search format: Mpepseq(pep. Sequence) Example: 1222.66 seq(LIEDNEYTAR)

  4. Exercise 3. MS/MS manual spectrum interpretation In the raw file go to scan number: 10194 Move to the corresponding MS1 scan to determine the charge state and peptide mass Solve the MS/MS spectrum Use the peptide mass and sequence for a Sequence search using: http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=SQ Search format: Mpepseq(pep. Sequence) Example: 1222.66 seq(LIEDNEYTAR)

  5. Exercise 4. MS/MS manual spectrum interpretation In the raw file go to scan number: 7726 Move to the corresponding MS1 scan to determine the charge state and peptide mass Solve the MS/MS spectrum Identify the peptide modification Use the peptide mass and sequence for a Sequence search using: http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=SQ Search format: Mpepseq(pep. Sequence) Example: 1222.66 seq(LIEDNEYTAR)

  6. Exercise 2. MS/MS manual spectrum interpretation In the raw file go to scan number: 12592 Move to the corresponding MS1 scan to determine the charge state and peptide mass Solve the MS/MS spectrum Identify the peptide modification Use the peptide mass and sequence for a Sequence search using: http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=SQ Search format: Mpepseq(pep. Sequence) Example: 1222.66 seq(LIEDNEYTAR)

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