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Prepare RNA mix in PCR tube 1 µg RNA 1 µl Random primer/poly dT mix 1 µl 10 mM dNTP Water to 12 µl

Prepare RNA mix in PCR tube 1 µg RNA 1 µl Random primer/poly dT mix 1 µl 10 mM dNTP Water to 12 µl Leave 5 ’ @ 65˚ C, then chill to 4˚ C Add 4 µl 5x first strand buffer 2 µl 100 mM DTT 1 µl RNAse inhibitor Leave > 2 ’ @ RT Add 1 µl Superscript III

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Prepare RNA mix in PCR tube 1 µg RNA 1 µl Random primer/poly dT mix 1 µl 10 mM dNTP Water to 12 µl

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  1. Prepare RNA mix in PCR tube • 1 µg RNA • 1 µl Random primer/poly dT mix • 1 µl 10 mM dNTP • Water to 12 µl • Leave 5’ @ 65˚ C, then chill to 4˚ C • Add • 4 µl 5x first strand buffer • 2 µl 100 mM DTT • 1 µl RNAse inhibitor • Leave > 2’ @ RT • Add 1 µl Superscript III • Leave 10’ @ 25 ˚ C, then 50’ @ 42 ˚ C • Inactivate by leaving 15’ @ 70˚ C • Use 1 µl for PCR with gene-specific primers

  2. Set up master mix for each primer combo on ice! • 1.1 µl 100x F primer (1 pMol/µl = 1µM final []) • 1.1 µl 100x R primer • 11 µl 10x PCR buffer • 2.2 µl 10 mM dNTP (200 µM final []) • 88.4 µl water • 0.7 µl Taq polymerase • Primers are • corn SOD or PEPC • Rice OEE or PHYA • Arabidopsis Actin and Aromatic Acid Decarboxylase • Add 19 µl to 1 µl correct cDNAs, 19 µl to 1 µl correct genomic DNA, 19 µl to 1 µl no template cDNA and 19 µl to 1 µl water • Run 30 cycles of 15” @ 94, 50-1”/cycle @ 50, 15” @ 72

  3. Possible Projects Studying interactions between plants and Geobacter (or other electrogenic bacteria)

  4. Possible Projects • Studying interactions between plants and Geobacter (or other electrogenic bacteria) • Studying interactions between • cyanobacteria and Geobacter • (or other electrogenic bacteria)

  5. Possible Projects Studying interactions between plants and Geobacter (or other electrogenic bacteria) Studying interactions between cyanobacteria and Geobacter (or other electrogenic bacteria) Studying potential of Rhodopseudomonas (a.k.a. Rhodobacter) for biophotovoltaics

  6. Possible Projects • Studying interactions between plants and Geobacter (or other electrogenic bacteria) • Studying interactions between cyanobacteria and Geobacter (or other electrogenic bacteria) • Studying potential of Rhodopseudomonas (a.k.a. Rhodobacter) for biophotovoltaics • Switch to photosynthesis when go anaerobic!

  7. Possible Projects • Studying interactions between plants and Geobacter (or other electrogenic bacteria) • Studying interactions between cyanobacteria and Geobacter (or other electrogenic bacteria) • Studying potential of Rhodopseudomonas (a.k.a. Rhodobacter) for biophotovoltaics • Switch to photosynthesis when go anaerobic! • Selection for cells that can transfer e- to electrodes!

  8. Possible Projects Studying interactions between plants and Geobacter (or other electrogenic bacteria) Studying interactions between cyanobacteria and Geobacter (or other electrogenic bacteria) Studying potential of Rhodopseudomonas (a.k.a. Rhodobacter) for biophotovoltaics Studying Cyanobacteria for biophotovoltaics

  9. Possible Projects • 4. Studying Cyanobacteria for biophotovoltaics • Finding new ones

  10. Possible Projects • 4. Studying Cyanobacteria for biophotovoltaics • Finding new ones • Tweaking knowns • http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Protein-Expression-and-Analysis/Protein-Expression/algae-engineering-kits.html

  11. Possible Projects • 4. Studying Cyanobacteria for biophotovoltaics • Finding new ones • Tweaking knowns • Tweaking nanowires

  12. Possible Projects • 5. Studying green algae for biophotovoltaics • Finding new ones • Tweaking knowns(Chlorella, Dunaliella)

  13. Possible Projects • 6. Studying plants for biophotovoltaics • Roots under hypoxia

  14. Possible Projects • 6. Studying plants for biophotovoltaics • Roots under hypoxia • Aquatics under CO2 deprivation

  15. Possible Projects • 6. Studying plants for biophotovoltaics • Roots under hypoxia • Aquatics under CO2 deprivation = every day!

  16. Possible Projects • 6. Studying plants for biophotovoltaics • Roots under hypoxia • Aquatics under CO2 deprivation = every day! • Some aquatics do CAM because CO2 is low in water in the day

  17. Possible Projects • 6. Studying plants for biophotovoltaics • Roots under hypoxia via mediator • Aquatics under CO2 • deprivation • Some aquatics do CAM • because CO2 is low in water • in the day • Perhaps others will • transfer electrons to anodes • during the day to dissipate • excess energy

  18. Possible Projects 7. Engineering the production of other novel products

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