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EGFR-DN and c-Jun Regulation of Luc Activity in Response to LPA and AG1478 Treatment

This study investigates the role of Epidermal Growth Factor Receptor Dominant Negative (EGFR-DN) and c-Jun in modulating luciferase activity under various conditions involving lysophosphatidic acid (LPA) and AG1478 treatment. The experimental design includes multiple controls, including LPA, HGF, and their inhibitions, assessing the pathways and interactions involved in cell signaling. Key findings reveal the activity of c-Fos and Jun-B in response to these treatments, providing insights into cellular responses influenced by growth factor signaling in a multi-factorial environment.

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EGFR-DN and c-Jun Regulation of Luc Activity in Response to LPA and AG1478 Treatment

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  1. LPA/HGF/AG1478 LPA LPA/AG1478 3xAP-1 Luc TK 30 20 DMSO AG1478 10 A 0 0.5 1 2 4 6 0 0.5 1 2 4 6 0 0.5 1 2 4 6 hr c-Jun 1 3.9 14.7 17.8 8.2 2.7 1 1.4 2.0 4.4 4.7 2.7 1 2.7 10.9 13.1 7.6 2.6 c-Fos vector EGFR-DN 1 23 63.7 68.6 44.5 18.5 1 2.3 8.8 11.2 2.2 1.1 1 21.3 52.6 54.2 36.5 15.4 EGFR-DN Ade control Ade Fra-1 LPA 0 2 0 2 hr LPA 0 0.25 0.5 1 2 0 0.25 0.5 1 2 hr 1 0.7 1.1 11.6 40.9 43.2 1 0.9 1.1 1.4 3.0 1.8 1 1.2 1.5 10.3 39.3 38.8 Jun-B c-Jun c-Jun 1 1.4 1.8 6.5 14.9 10.8 ND ND ND ND ND ND 1 1.2 1.6 5.7 14.7 14.7 c-Fos GSK-3a/b c-Fos EGFR Jun-B EGFR-DN Luc activity (fold) Fra-1 b actin EGFR * B EGFR-DN b actin LPA LPA control control C LPA LPA/AG1478 LPA/HGF/AG1478 0 0.5 1 2 4 0 0.5 1 2 4 0.5 1 2 4 hr

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