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HC70AL Research Presentation

This research presentation by Eric Newton investigates the phenotypic outcomes of two genes, AT3G47640 and AT3G61310, in various plant genotypes including wild types and mutants. The study highlights the differences in expression and phenotype, with AT3G47640 displaying a clear mutant phenotype while AT3G61310 lacks visible traits. The investigation uses a comparative approach contrasting data from the Salk online database and lab results, including promoter region analysis. Overall, insights into gene expression timing and locations are discussed, alongside the challenges faced in the cloning process.

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HC70AL Research Presentation

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Presentation Transcript

  1. HC70AL Research Presentation Eric Newton

  2. First gene AT3G47640Wild Type: 1,409Mutant: 730

  3. Second gene AT3G61310Wild Type: 621Mutant: 609

  4. First gene plants genotype Plant 1: HeterozygousPlant 2: WTPlant 3: WTPlant 4: MutantPlant 5: WTPlant 6: WTPlant 7: WTPlant 8: -undetermined-Plant 9: MutantPlant 10: WTPlant 11: MutantPlant 12: -undetermined-

  5. Gene 1 mutant plant phenotype Why no phenotype for 2nd gene?

  6. Second gene plants genotype

  7. Salk vs. my results Gene anatomy according to Salk online databaseGene anatomy according to my lab results

  8. When and where are the genes expressed? Second Gene First Gene

  9. Promoter region 1 What were the sequence results? 1-kb Ladder Clone #1 Clone #2

  10. Promoter region gene 2 What were the sequence results?

  11. Gene 1-found mutant-no visible phenotype-Promoter cloning obsolete Gene 2-no found mutant-phenotype N/A-Promoter cloning has mutations Conclusion

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