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Scheme of H 4 MPT-dependent C1 carbon compound conversion steps. C1 substrate. HCHO. H 4 MPT. Fae (formaldehyde activating enzyme). CH 2 = H 4 MPT. NADP +. MtdC (methylene-H 4 MPT dehydrogenase). NADPH . CH ≡ H 4 MPT +. Mch (methenyl-H 4 MPT cyclohydrolase). CHO-H 4 MPT. “MFR“.
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Scheme of H4MPT-dependent C1 carbon compound conversion steps C1 substrate HCHO H4MPT Fae (formaldehyde activating enzyme) CH2=H4MPT NADP + MtdC (methylene-H4MPT dehydrogenase) NADPH CH≡H4MPT+ Mch (methenyl-H4MPT cyclohydrolase) CHO-H4MPT “MFR“ Ftr (formylmethanofuran:H4MPT-formyltransferase) H4MPT CHO-“MFR“ Fmd(ABC) (formylmetanofuran dehydrogenase) HCOOH