1 / 3

His-SUMO1

Supplemental Fig.1. a. b. V. PML. HA-PML. -. +. +. +. +. His-SUMO1. +. +. -. +. -. +. ++. +. ++. His-SUMO2. +. +. +. +. +. GST-SUMO1. -. -. -. -. +. +. +. -. +. Flag-c-Jun. Mdm2- SUMO2. Ni beads. WCL. Mdm2. c-Jun-His -SUMO1. 75. Ni beads. 1. 2. 3.

alyson
Télécharger la présentation

His-SUMO1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Supplemental Fig.1 a b V PML HA-PML - + + + + His-SUMO1 + + - + - + ++ + ++ His-SUMO2 + + + + + GST-SUMO1 - - - - + + + - + Flag-c-Jun Mdm2- SUMO2 Ni beads WCL Mdm2 c-Jun-His -SUMO1 75 Ni beads 1 2 3 4 50 c 37 His-SUMO1 c-Jun IB c-Jun- SUMO1 HA-PML - + - + 75 100 50 c-Jun- SUMO1 75 c-Jun WCL 37 PML- SUMO1 50 100 c-Jun 75 PML IB 37 GFP 1 2 3 4 1 2 3 4 5

  2. Supplemental Fig.2 a PML 150 150 100 PML 75 100 PML 50 75 actin 37 * 25 50 15 coomassie blue staining Western blot b TRIM27 Mdm2 - 100 - 75 100 Mdm2 75 Trim27 * - 50 c GST-TRIM27 GST-PML GST GST-TRIM27 GST-PML GST 1 2 3

  3. Supplemental Fig. 3 a Trim22S Trim22L Trim11 Trim22L V Trim27 V Trim39 His-SUMO1 + + + - + + + - + - + + + + HA-Mdm2 + - + + - + + + + + - + + - 150 Mdm2- SUMO1 Ni beads 100 100 Mdm2 WCL Actin GFP b Trim27 V Trim24 Trim25 V Trim18 Trim1 V + + + ++ + ++ + ++ + ++ + ++ + ++ 150 Ni beads Mdm2- SUMO1 100 100 Mdm2 WCL Actin GFP c UbcH5a - + + + TRIM27 + + + - 82 p53-Ub 64 p53 p53 1 2 3 4

More Related