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Lab 8

Lab 8. Bacterial Growth Curve. VIABLE CELL COUNTS DILUTION PLATING 10-FOLD DILUTION SCHEME. BACTERIAL GROWTH CURVE. SPECTROPHOTOMETRY. ACTUAL DATA Using absorbance to measure turbidity of culture Dilution plating to measure the viable no. of cells G T extrapolated from the graph.

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Lab 8

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  1. Lab 8 Bacterial Growth Curve

  2. VIABLE CELL COUNTS DILUTION PLATING 10-FOLD DILUTION SCHEME

  3. BACTERIAL GROWTH CURVE

  4. SPECTROPHOTOMETRY • ACTUAL DATA • Using absorbance to measure turbidity of culture • Dilution plating to measure the viable no. of cells • GT extrapolated from the graph

  5. VIABLE CELL & OPTICAL COUNTS

  6. VIABLE CELL COUNTS DILUTION PLATING 10-FOLD DILUTION SCHEME 100 ul 100 ul 100 ul 100 ul 100 ul 900 ul 4 6

  7. Procedure • Measurement of OD and dilution and plating of samples from an E. coli culture every 30 min. Plate 100 ul of appropriatedilution Serial dilutions Take100ul Measure OD Take 1000 ul Spread Culture CFU/ml = No. of colonies x dilution / plated vol.

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