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Exploring Soil Microbial Diversity Across Seasons and Depths at Rothamsted Park

This study conducted at Rothamsted Park analyzed soil samples from different depths across various seasons between February 2009 and July 2010. DNA extractions were performed using indirect and direct methods to investigate microbial communities, focusing on Gram-positive bacteria. Techniques like MP Bio1O1, MoBio kits, and various lysis methods were employed for analysis, particularly in the rhizosphere. The study provides valuable insights into the microbial diversity present in the soil at Rothamsted Park.

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Exploring Soil Microbial Diversity Across Seasons and Depths at Rothamsted Park

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  1. Rothamsted Park Grass soil Seasons February2009 July 2009 July 2010 Soil depths 0-21 cm 0-21 cm 0-10 cm 11-21 cm 0-21 cm 0-10 cm DNA extractions Indirect Gram positive Direct MP Bio1O1 Indirect MP Bio1O1 Direct MP Bio1O1 (Rhizosphere) Indirect DNA Tissue Indirect DNA Tissue Indirect MP Bio1O1 Indirect lysis in plug Indirect lysis in plug Direct MP Bio1O1 Direct MP Bio1O1 Direct MP Bio1O1 Direct MoBio F1 F2a F2b F3 F4 F5 F6 J1 J7 J4 J4 J1a-10 J1b-10 J1rhizo-10

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