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Rotating Vessel Wall For the Adhesion of Cardiac Cells

Rotating Vessel Wall For the Adhesion of Cardiac Cells. Roli Kumar BME Oral Presentation#1 Advisor: Dr. Marc Lin. Background. Many surgical and radiational procedures have left areas of damaged tissue in the heart. Test new drugs study development and function of cardiac tissue. Objectives.

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Rotating Vessel Wall For the Adhesion of Cardiac Cells

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  1. Rotating Vessel WallFor theAdhesion of Cardiac Cells Roli Kumar BME Oral Presentation#1 Advisor: Dr. Marc Lin

  2. Background • Many surgical and radiational procedures have left areas of damaged tissue in the heart. • Test new drugs • study development and function of cardiac tissue

  3. Objectives • 3-D cardiac tissue from a monolayer of cardiac cells • 3-D cell cultures contain specifications similar to in vivo: • cell to cell and cell to matrix interactions • composition of interstitial fluid • Three dimensional Cultures of Prostatic Cells

  4. Design Specifications of Rotating Vessel • End over end mixing of the cells • Continuous rotation of the vessel • Max of .2 dyne/cm2 of shear stress • Minimal fluid turbulence • Bubble-free aeration • eliminate hydrodynamic forces • Solid Body Rotation • Suspending the cells in a liquid medium.

  5. Apparatus Set-Up Plastic Rod Rotating vessel Water Magnetic Stirrer

  6. Work Completed • Literature search was completed: • Cell disassociation • Rotating-Wall Vessel for Prostate Cells • Found a metal ball

  7. Current Work • I am meeting with Dr. Bader who does Cardiology research to learn how to disassociate and cardiac cell growth. • Putting the apparatus together

  8. Future Plan of Action • Calculate the sheer stress applied on cells when rotated • Literature search on a way to bubble-free aeration of cells while they are rotating

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