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Toxicology Using Daphnia, ViBRIo and Nitrate Solution

Toxicology Using Daphnia, ViBRIo and Nitrate Solution. Victoria McLain Briana Price Austin Graves Chris Forcinito Gideon Whaley Josh Taylor Sam Holden. Can Daphnia Magna and Vibrio Fischeri be used in a quick and simple nitrogen test?. MATERIALS (Daphnia).

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Toxicology Using Daphnia, ViBRIo and Nitrate Solution

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  1. Toxicology Using Daphnia, ViBRIo and Nitrate Solution Victoria McLain Briana Price Austin Graves Chris Forcinito Gideon Whaley Josh Taylor Sam Holden

  2. Can Daphnia Magna and VibrioFischeribe used in a quick and simple nitrogen test?

  3. MATERIALS (Daphnia) • 4 100 ml graduated cylinders • 4 storage bottles • 4 flasks • Sodium Nitrate (s) • Sharpie • Centigram Balance • Weigh boats • Tape

  4. PROCEDURE • Fill graduated cylinders to 100 ml of dH2O • Weigh 10 g of Sodium Nitrate • Add the 100 ml of dH2O into flask • Add 10 g of Sodium Nitrate to flask Mix well • Put in storage container and label concentration • Repeat procedure with varying weights of Sodium Nitrate depending on wanted concentration • Percent solution= (dry mass in grams/ volume in mL) *100

  5. Number of Days to death of sample population (x) Parts per million (y)

  6. CONCLUSION • Over the course of this experiment, we did indeed test the Daphnia. However, we do not believe that the data that we collected is exclusive enough to answer the question that was posed. • We only tested a few sets of Daphnia. • We were not able to test them in increments of minutes, but days. • The Daphnia took a substantially long amount of time to produce evidence, or change.

  7. Materials (Vibrio) • Slants containing bacteria. • Test tubes containing broth. • Heat source. • Incubator. • Plastic rods. • Petri Dishes. • Small paper discs.

  8. VibrioFischeri We have concluded that, as per our original research question about the efficiency of using VibrioFischeri for nitrogen testing, VibrioFischeri is not an expeditious tester. We found the following issues: • Growth time • Obscure effects • Saltwater vs. Freshwater • Fragile

  9. WORKS CITED • http://upload.wikimedia.org/wikipedia/commons/c/c0/Daphnia_magna.png • http://slowmuse.files.wordpress.com/2009/02/daphnia-15551.jpg • https://wildco.com/images/pctr/4120-A10.jpg • http://dl.clackamas.cc.or.us/ch104-01b/images/104vol01.jpg

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