Investigating Protein Interactions with Bait Sequences in Transcription Assays
This study examines the interactions of hybrid proteins with specific bait sequences using reporter gene assays. We focus on the interaction of the bait sequence with transcription factors that drive expression of AD.TATA.HIS3 and MEL1.UAS reporter genes. We assess both specific and non-specific protein interactions, utilizing the minimal GAL4 promoter. Selection for positive growth on CM–His–Leu media supplemented with 3-AT and X-GAL allows for the identification of successful interactions. This work contributes to our understanding of protein-DNA interactions and transcriptional regulation.
Investigating Protein Interactions with Bait Sequences in Transcription Assays
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Presentation Transcript
Transcription if hybrid protein interacts with bait sequence Library protein AD TATA HIS3 reporter gene Bait sequence Transcription if non-specific protein interacts with the minimal GAL4 promoter TATA MEL1 reporter gene MEL1 UAS Non-specific protein AD Selection for positive growth on CM –His –Leu 3-AT and X-GAL plates
