MnSOD from E. coli PDB structure 1VEW

1. Structure of Active site of MnSOD from E. coli Free and bound to Azide (N3¯) Gln 145 Trp 168 Tyr 34 OH Cu N3¯ His 170 Thr 33 His 30 MnSOD from E. coli PDB structure 1VEW Azide bound to MnSOD from E. coli PDB structure 1MNG The bound N3¯ anion suggests how O2¯approaches the active site.

2. Orientation of azide differs in structures of Fe- and MnSOD from E. coli N3¯ Azide bound to FeSOD from E. coli PDB structure 1ISB Azide bound to MnSOD from E. coli PDB structure 1MNG Azide chanes orientation in Fe structure, raises question about active site flexibility.

3. The active site of FeSOD from M. tuberculosis is very similar to that of MnSOD from E. coli His 145 Gln 145 OH Cu His 170 FeSOD from M. tuberculosis PDB structure 1IDS MnSOD from E. coli Similarity of structures suggests that inhibitors of E coli MnSOD could be used against M. tuberculosis.

4. Comparison of CuZnSOD active sties with and without azide Azide complexed Bovine CuZnSOD Human CuZnSOD PDB 1SXZ PDB 1PU0 Asn 139 Glu 133 Gly 139 Glu 133 Lys 136 Ser 140 Lys 136 Arg 141 Pro 62 Pro 62