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IVOS & CEROS Computer Assisted Sperm Analysis

IVOS & CEROS Computer Assisted Sperm Analysis. Spermatology 2006. What is CASA?. An automated system designed to: Visualize and digitize successive images of sperm Process and analyze the images Provide precise and meaningful information on sperm kinematics and counts

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IVOS & CEROS Computer Assisted Sperm Analysis

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  1. IVOS & CEROSComputerAssistedSpermAnalysis Spermatology 2006

  2. What is CASA? • An automated system designed to: • Visualize and digitize successive images of sperm • Process and analyze the images • Provide precise and meaningful information on sperm kinematics and counts • Combination of hardware and software • Hardware: optics, illuminator, digitizer, computer • Software: modules for motility, morphology, and viability analysis

  3. 2 Current CASA Models from HT • IVOS Sperm Analyzer • Built-in optical system • Automated, heated stage • Fastest analysis: 6 fields in 6 seconds • CEROS Sperm Analyzer • Complete system • Includes negative phase contrast microscope

  4. NEW!! Modular CEROS • Choose onlythe options you need • Cost-effective • Use your own negative or positivephase contrast microscope • Same proven and trustedanalysis algorithms

  5. Multi-species Compatibility • Human • Non-human primates • Domestic / companion animals • Production animals • Endangered species • Laboratory rodents • Sea mammals and fish

  6. Customized CEROS for Fish Sperm • “Cooled” stage for optimum maintenance of fish sperm motility • Applications include: • Monitoring sperm motility at hatcheries • Testing extension media and spermiation stimulant • Monitoring environmental pollutants • Reproductive toxicology • Fish, sea urchin, etc.

  7. Negative Phase Contrast - Standard 10x Negative Phase 20x Negative Phase Provides fastest analysis Provides superb visualization

  8. Getting Ready for Analysis (IVOS) • Load sample in fixed or known depth chamber (e.g. Leja Chamber, Microcell, Makler) • Place filled chamber on stage • Select multiple analysis fields • Press Start Scan

  9. Sperm Analysis • Captures multiple “snapshots” extremely quickly (e.g., 30 images in 0.5 second). • Tracks the motile cells from snapshot to snapshot • Static sperm are identified based on motile cell means (size, contrast, intensity) • Computer calculates results

  10. Results • Motility • Progressive Motility • Concentrations • Counts • Velocity - 3 types • Motion parameters

  11. Playback for Quality Control • Motile sperm marked with green track • Progressive sperm marked with blue track • Slow sperm marked with purple track • Static sperm marked with red dot

  12. Motile Playback Sample

  13. Research Options • Obtain data on individual tracks (Edit Tracks) • Study sub-populations of sperm (Sort)

  14. Data Management • Clinical Filing • Custom designed reports • Store reports to disk • HDATA ASCII Export • Export data in database / spreadsheet compatible format • Summary and individual track data

  15. Digital Image Storage • Ability to save all fields analyzed • Recall and reanalyze fields • Sequential field analysis – all fields from original analysis automatically analyzed in order • Share images with other IVOS/CEROS users • Create QC images for training purposes

  16. Morphology Options (Automated) • Dimensions Morphology • Human sperm only • Analysis based on Dr. Kruger’s Strict Criteria • Diff-Quik and PAP stains • Metrix Morphology • Multi-species • Analysis based on user’s definition of normal • Diff-Quik, PAP and other high contrast stains

  17. Real-Time Morphology (non-automated) • Subjective (visual classification) • Fast – “REAL-TIME” • Use non-stained, immobilized sperm, or standard stained smear • Results combined with motility results • Morph-merge option

  18. IDENT Fluorescence (IVOS Only) • Static and motile sperm identification for high-detritus samples • Sperm stained with Hoechst 33342 stain • All sperm fluoresce under UV light • Accurate counts to within 1%

  19. VIADENT Fluorescence (IVOS Only) • Sperm stained with Hoechst 33258 stain • Only NON-VIABLE sperm fluoresce under UV illumination • Counting • Motile/Total counts under standard illumination • Viable count under fluorescent illumination • Only system to perform motility and viability analysis on the same, live sample

  20. Benefits of CASA • Objectivity: Removes technician bias • Accuracy: Best method for determining percent motility, percent progressive motility, and velocity parameters • Precision: Repeatable results • Sensitivity: Detects even the slightest changes in sperm motion • Reliability: Does not get “tired eyes” – can analyze samples all day without sacrificing accuracy

  21. Hamilton Thorne Contact Information • Phone: 978-921-2050, 800-323-0503 • Fax: 978-921-0250 • E-mail: info@hamiltonthorne.com • Web: www.hamiltonthorne.com • 100 Cummings Center, Suite 465EBeverly, MA 01915 USA

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