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DNA Analysis in Crime Scene Investigations: Key Connective Questions and Troubleshooting Tips

In this engaging question-based approach, we explore how to connect suspects to crime scenes through DNA evidence analysis. Understanding how to interpret gel electrophoresis results is crucial for forensic investigations. If you encounter unusual results, common issues may arise from incorrect buffer concentrations, gel preparation errors, or gel runtime inconsistencies. This guide includes sample questions, vocabulary sheets, practice problems, and troubleshooting techniques for various lab scenarios. Dive into the essential tools and practices for accurate DNA analysis in criminal investigations.

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DNA Analysis in Crime Scene Investigations: Key Connective Questions and Troubleshooting Tips

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  1. Crime Scene DNA: Question of the Day #2 #1 Question: Which suspect can you connect to the crime scene & how do you know? Answer: … … … • 1’s Turn In: • Vocab Sheet • Practice Problems • Class Sets for ANY Lab

  2. Scenario 2 Scenario 1

  3. Scenario 2 Scenario 1

  4. Sample Results:

  5. Sample Results:

  6. Gel Troubles? • Unusual gel results can occur as a result of the following conditions: - Making your gel with water or the wrong concentrations of buffer. - Overloading the well. - Running your gel too fast or too slow. - Puncturing the gel when loading. • A bubble forms in a lane of your agarose gel. • Pulling combs out of gel before it has solidified. Gel made with water and agarose instead of buffer.

  7. *Gel loaded with too much sample. *A gel run too short so there is poor separation between the bands. *A gel run too long. More Gel Troubles

  8. Comb pulled out too early. *Punctured wells *A bubble set in the agarose in lane 1. More Gel Troubles

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