1 / 15

Laboratory Methods in Biotechnology

Laboratory Methods in Biotechnology. Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety. Idea Behind the Course.

beryl
Télécharger la présentation

Laboratory Methods in Biotechnology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Laboratory Methods in Biotechnology Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety

  2. Idea Behind the Course • Provide practical experiences in laboratory activities that will help students make the transition from content-based lower division courses to application orientated senior level courses. • Provide skills and dispositions needed to carry out laboratory research as part of a career in the life sciences. This includes undergraduate research, working as a technician, graduate research, and affiliated professions.

  3. Role in Your Curriculum Core: Reasoning, Investigation, Cell Biology, Genetics, MCMB Specialty: Lab Methods, Micro, Molecular Genetics Electives: Molecular Techniques, Cell and Molec, Immunology, Path Micro, Microbial Phys, etc

  4. Objectives • To give students experience in dimensional analysis used in microbiology and cellular and molecular biology, and biotechnology

  5. Objectives • To familiarize the students with the theory and practice of preparing solutions from various starting materials.

  6. Objectives • To introduce the students to means of preparing sterile media and solutions.

  7. Objectives • To allow students to practice quantifying bacteria and viruses, and to measure the growth rates of bacterial populations.

  8. Objectives • To allow students to quantify proteins, nucleic acids and other chemicals in solution.

  9. Objectives • To introduce students to commonly used devices including, but not limited to: microliter pipetters, centrifuges, spectrophotometers, pH meters, etc.

  10. Objectives • To introduce the students to the use of enzymes for end-point determination and to the measurement of enzyme activity.

  11. Objectives • To give students experience in the techniques for the manipulation and electrophoretic separation of proteins and DNA.

  12. Objectives • To provide information regarding biological, chemical, radiation, and other aspects of lab safety.

  13. Textbooks • Make sure you bring this each time. • Seidman and Moore – our main text • Leboffe and Pierce – illustration of techniques • Also need: • Calculator! • Lab Notebook

  14. Additional Resources • Brock – background information on theory • Day – advice on writing (humor) • Robyt - Source of many handouts/additional readings • CBE Manual – Style guide for preparation of manuscripts • Don’t buy these

  15. Webpagehttp://cstl-csm.semo.edu/champine/BI245/index.html • Lab Activities • Lecture Topics • Homework Assignments • In Class Assignments • Manuscripts • Notebook Guidelines • Syllabus • Grade Book

More Related