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Biotechnology Summer Research Program Jeanne Smith – Olympic High School

Biotechnology Summer Research Program Jeanne Smith – Olympic High School. Olympic High School, Charlotte, NC. Large School divided into 5 smaller , theme-based schools Biotechnology, Health & Public Administration Students involved in program: Rising 9 th – 12 th grade students

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Biotechnology Summer Research Program Jeanne Smith – Olympic High School

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  1. Biotechnology Summer Research ProgramJeanne Smith – Olympic High School

  2. Olympic High School, Charlotte, NC • Large School divided into 5 smaller, theme-based schools • Biotechnology, Health & Public Administration • Students involved in program: Rising 9th– 12th grade students • School Demographics: • 52.8% African American • 17.0 % Hispanic • 15.8% Caucasian • 10.1% Asian • 0.9% American Indian • 3.3% Others • Students participating in the school’s subsidized lunch program: 52%

  3. Biotechnology Summer Research Program • GetBiotechSmart’s Excellence in Biotechnology Grant • Objectives: • To introduce andapply biotechnology scientific concepts to laboratory experiments; • To stimulate student interest in pursuing biotechnology in college and careers; • To involve students in scientific research from sample collecting, laboratory work & computer analysis.

  4. National Science Standards – Unifying Concepts • Students combine processes and scientific knowledge with scientific reasoning and critical thinking to develop their understanding of science. • Life Sciences focuses on scientific facts, principles, theory and models that are important: the cell & molecular basis of heredity • An understanding of science and technology establishes connections between the natural and designed world, linking science and technology. • A personal and social perspective of science helps a student understand and act on personal and social issues.

  5. Summer Research Program • B-3 Program: Biotechnology, Biodiversity, Bioinformatics • Research tied to a regional environmental issue • Partnerships • Content support through State Parks, University of North Carolina at Charlotte, and American Chestnut Foundation • Hands-on Experience • Field sampling for students to see the environmental issue • Laboratory experience • Involved Parents • Orientation meeting • Student presentations to families

  6. Our Students – Rising 9th – 12th Grade Students

  7. American Chestnut – Regional Environmental Issue A fungal blight has wiped out a major deciduous tree in Eastern US American Chestnut tree was an important resource in NC: furniture, buildings, animal feed

  8. Field Sampling - Collecting Leaf Samples for Research

  9. Hands-on Laboratory Experience

  10. Hands on Laboratory Experience

  11. Steps to isolate the DNA for PCR & analysis

  12. Partners through the local University & State Park Ranger Kelly Cook, NC State Parks Dr. Jennifer Weller, UNCC Graduate Students Shatavia Morrison Chris Overall

  13. Concluding Presentation to Families

  14. Grant Support Enabled Our Biotech Research • Expands the Summer Research Program • Two Levels of Laboratory Experience • First level: biotechnology skills based program • Second level: biotechnology research focus to compare DNA of American Chestnut tree and Chinese Chestnut tree (blight resistant) • Allows 30 students to participate • Key equipment – • Supplement current equipment with micro-centrifuge, vortex mixers, digital balance, gel documentation • Disposable Supplies to support • Horizontal (agarose) and vertical (acrylamide) gel electrophoresis • Polymerase chain reaction

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