Shaniqua Andrews, Crystal Hill, and Dominique Saddler (Bioengineering program, CBBE-NCAT),

1. Characterization of Gold Nanoparticles Using Dynamic Light Scattering for Biomedical Applications-BMEN 570 Shaniqua Andrews, Crystal Hill, and Dominique Saddler (Bioengineering program, CBBE-NCAT), Dr. N. Bhattarai NCAT-Advisor Introduction Results • Gold nanoparticles (Au-Np) have unique optical and electronics properties. • Au-Np have been wildly researched and utilized in high technology applications such as organic sensors, therapeutic agents, drug delivery in medical applications, electronic conductors and catalysis. • Properties Au-Np are size dependent Figure 1. Observed color changes as the gold chloride solution went through the growth reaction. • H[AuCl4]Ÿ3H2O (0.2% w/v) seed particles large goldparticles Research Goals • Synthesis of colloidal gold nanoparticles and characterize them by using Dynamic Light Scattering (DLS) technology: • Enhance the understanding of nanoparticle synthesis techniques by chemical method. • Measurement of diameter of Au-Nps. • Understanding and observing principle nanoparticle properties Methods • Nanoparticle Synthesis: • Synthesis of the seed • Reaction mechanism: reduction of gold(III) via ascorbic acid • Sodium citrate as stabilizer • Growth of seeds • Seed solutions were added together within 5mins. • Final solution was boiled then refrigerated Figure 2. DLS analysis of gold nanoparticles (Au-Nps) • DLS Method: • Create protocol: • Refraction index • Dispersant • Temperature • Cuvette type • 2. Run protocol: • 2-5mins., with < 1 mL sample • Size distribution data produced Conclusion • Successful in synthesizing uniform gold-seed particles • Aggregation evident in gold nanoparticles: • Added step of shaking • Confirmed by DLS • Further application: drug delivery Acknowledgements • Funding Support from NSF’s NUE at NCAT • Mr. Roman Blount IV for helping in lab • Dr. Shyam Aravamudhan and Mr. Karshak Kosaraju f or helping in DLS