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Multilocus Sequence Typing of bacteria: a genotypic tool for strain selection

Multilocus Sequence Typing of bacteria: a genotypic tool for strain selection Aundy Kumar, *Jos M. Raaijmakers, Santhosh J Eapen Indian Institute of Spices Research, Calicut ___________________________________________________________________

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Multilocus Sequence Typing of bacteria: a genotypic tool for strain selection

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  1. Multilocus Sequence Typing of bacteria: a genotypic tool for strain selection Aundy Kumar, *Jos M. Raaijmakers, Santhosh J Eapen Indian Institute of Spices Research, Calicut ___________________________________________________________________ **Laboratory of Phytopathology, Wageningen University, The Netherlands Image courtesy: pseudomonas.com

  2. Tools and web resources at your door step! Advances in sequencing methods Ever reducing cost of sequencing Freely available analytical tools Good databases with constant updates Full genome information on 1400 bacteria http://pubmlst.org/ http://www.mlst.net/ http://www.pasteur.fr/recherche/genopole/PF8/mlst/

  3. Pseudomonas Acinetobacter Klebsiella Stenotrophomonas Enterobacter Bacillus Burkholderia Frequently encountered ones MLST scheme has been developed for ~40 bacteria so far

  4. What is MLST? • A nucleotide sequence based approach to uncover allelic variants in conserved genes (house keeping) for the purpose of characterizing, subtyping, and classifying members of bacterial populations. • Proposed in 1998 using the human pathogen Neisseriameningitidis as an example • A portable, universal, and definitive method for characterizing bacteria

  5. MLST – genes used

  6. PROTOCOL PCR amplification of selected genes Elution & purification of PCR product Sequencing of PCR product Sequence assembly (DNA Baser) Blast the assembled sequence in database www.PAMDB.org Assigning allele numbers for each gene Unique allelic profile is called the Sequence Type(ST)

  7. Each sequence for a given locus is screened for identity (not for similarity) with already known sequences for that locus. If the sequence is different, it is considered to be a new allele and is assigned a unique (arbitrary) allele number

  8. Case study of Multilocus Sequence Typing of plant associated Pseudomonas aeruginosa

  9. In Spice crops Projects on bacterial endophytes in black pepper/ginger against major soil borne pathogens …has yielded several diverse group of bacterial species The best among the lot is Pseudomonas aeruginosa Aravind et al Letters in Applied Microbiology, 2009

  10. …background Selected from several collection in various projects during different points Some of them are endophytes in plants

  11. DNA extracted from bacterized stem is used as template DNA of BP35 BP35 Density in Suspension 8-10 log units 578bp Phytophthora Suppression 90-100% Endogenous Population of BP35 4.5-6.5 log units Phenotype- functional Direct evidence for protection of black pepper by specific density of P. aeruginosa BP35 cells

  12. Roots Stem Leaf Phenotype- functional Accumulation of bacterial cells The bacterium was tagged with gpf using Tn7 system Localization of BP35::gfp2 in tomato Tracking of bacterium PaBP35 15 Days Post Bacterization in Five weeks old tomato cultivar, Moneymaker Tissues fixed in 4% paraformaldehyde Localized in aerial plant parts

  13. Few of them are in field evaluation stage for Phytophthora suppression Radopholus similis suppression March 2010 July 2010 August 2010 Single or Two nodal cutting are Bioprimed Metalaxyl Bacterized shoots

  14. Collectively they may be classified as Plant Associated Pseudomonas aeruginosa Pseudomonas aeruginosa- Type strain for Pseudomonas Most intensively studied bacterium Opportunistic human pathogen Full genome published in Nature

  15. MLST analysis Analysis of housekeeping genes that are scattered in the genome www.pseudomonas.com

  16. guaA mutL nuoD ppsA Choice of genes for MLST analysis acsA aroE M: DNA ladder 50bp trpE Genes were amplified using specific primers The amplicon were eluted using gel elution kit (Sigma Aldrich) Sequenced (Both forward and reverse run)

  17. trpE ppsA nuoD guaA mutL acsA aroE trpE ppsA nuoD guaA mutL acsA aroE PAO1 BP35 Gene amplification, isolation, sequencing and sequence analysis 1000bp Out group from clinical origin

  18. guaA guaA guaA guaA guaA guaA mutL mutL mutL mutL mutL mutL nuoD nuoD nuoD nuoD ppsA ppsA ppsA ppsA nuoD nuoD ppsA ppsA aroE aroE aroE aroE aroE aroE acsA acsA acsA acsA acsA acsA All seven genes were isolated, sequenced and analysed trpE trpE trpE trpE trpE trpE IISR 6 IISR 51 IISR 13 BP35 IISR 853 GEB 9

  19. The sequences were assembled and analysed using Vector NTi Sequence viewer/ DNA baser

  20. Alleles were determined using pubmlst.org String of alleles (Sequence Type) were analysed using eBurst algorithm (eBurst V3) or profuse algorithm (goeBurst.org)

  21. >acsA-IISR6 TATAACTGTNGGNTTNAACGCGCTGACCCACGAGCGGGTCTTCGACTACCGTCCGGGCGAAGTCTACTGGTGCACCCGCCGGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCTGCAGCCAAGCCGTTCTTCGGCGTGGTACCGGCGCTGGTGGACAACCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTGGAAAGCGCGATGGTCGCCCACCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGGGAGCG Allele: 15 >aroE-IISR6 CATGTCAGATGGAAACCGATTGAGACGTACGTCTCAAATGCTGCCTGACAACTCAGACAGTTGCCAATTGCCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCACGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCGGTCGGTTCCTTGGCATACATCATGTCGTAGCAAACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGACCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGCGCACCGCGCCGAGATCGGCGAACCGCTCGGCCAGATCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAGCTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCGCGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAAGCCCGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCCAGGCGGATGAGGGTGTTCACCGCCCCGGCCCGGGTGGCCCGCTCGCTCAATTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGCTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGGGTGGGACGGGACGAGCGGACTCGTCCCCTGCGGGCGGGTCAGGGCTGGCCGGCCAGCCAATCGCGGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTTCCTCGCTGC Allele: 121 >guaA-IISR6 ANTNGNGGNCGNAACCNAANGNTGCCGCGGCCTGNCTTGGTGTTGGGTGACTTCCGGGGGGAATTCCAGCCGTTGNTGGCGCGGGAATTCGTNCCTTNAAGCGGTGGTGAGCCACGGCGACAAGGTCACCGAGATGCCGGCCGCGCTTCCACATCCTGGCCAGCACCCCGAGCTGCCCGATCGCCGCCATGGCAGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAAGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGTACCACAGACAGCCCGGGCGGTTCGATACANTGCNNCGCCTACNACGGCNGGCAATTCCGCCCNGAAGTCACTCACACAAGCANNNNGGCCANNCTCTGCCNGTTCCN Allele: 36 >mutL-IISR6 GGCGGTCGCGAGTCATGGTNGCGCGNACCGAACTAATCGAGCCAGCGCTCGCCACGGAGCCGNGGCTCATCCACCCGCCTTCAGAGANATGCGTCGCGTGAGTGGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTTGGTGGACGTCANNTGCACCCGAACNGCACGAGTTCGCTTNNNGACAGCCGGATGNCCATGACCTTCCCTCTNNNGAGANACCCNNNGA Allele: 5 >nuoD-IISR6 NTNNNGCCCACGCCAACTGGTACGAGCGCGAAGTGTGGGACATGTACGGCATCACCTTTCACCGGCCACCCGCACCTGACCCGCATGCTGATGCCGCCGACCTGGCAGGGCCATCCGCTGCGCAAGGACTACCCGGCGCGCGCCACCGAGTTCGATCCCTACTCCCTGTCCGCCGCCAAGCAGGACCTGGAGCAGGAAGCCCTGCGCTTCAAGCCGGAAGACTGGGGCATGAAGCGCCACGGCGAGAACGAGGACTACATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCATCCTGCAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACCGCGGCGCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACCGCATCGACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCTCGCCGGGATCAAGGTGCCCCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTTCCGTATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACAAGGTGGTCGAGGCCATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGCGGCGTCGCCCACGACCTGCCGCGCGGCTGGGACAAGCTGGTCCGCGAGTTCCTCGACTGGATGCCCAAGCGCCTCGACGAGTACGAGACCGCGGCCCTGAAGAACAGCATCTTGCGCGGCCGGACCATCGGCGTCGCCCAGTACAACACCAAGGAAGCCCTCGAGTGGGGCACCACCGGCGCCGGCCTGCGCGCCACCGGTTGCGACTTCGACCTGCGCAAGGCCCGCCCCTACTCCGGCTACGAGAACTTCGAGTTCGAGGTACCGCTGGCCCACAACGGCGACGCCTACGACCGCTGCATGGT Allele: 4 >ppsA-IISR6 AGATGCTTANGGAGCATAAGCTNTNGNAATCCTGGNNNCCCAANCCTCCTCCCTTCTATTNNNNGACGTCGATCGTNCGATCGCGCCNNTCGCCTGAGCGACGCCGAGGTCACCGAAGCTGGCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTTTCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCATGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAACGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCACCTGATCGGCGGCAGCTCTACGAGCAGGAGAAACATAACNCTCCACCAATGAANANANNGACNANN Allele: 15 >trpE-IISR6 ATCNNNTATTCAAGNCTCAATCGTTCAAGNACGATCCAGCNACAGCGNNNNNNGCNNNGCTTCCNCCTAATCNCGNNAAATNNGNNNNGCGCCNNCAGCNGNNNNGGCGCGNGCACGGCTGAAGGCAATGAACGCATGCATCGATCGCGTATCAAACGTAACTGCACTGCATGCGGGGGCGGATCGTGCCACCTCCGTCCCGGCGGCTTGTGATGGTTTCTTCCCACTCCAGCGCCGGCACCGAGTCGGCAACGATACCGCCGCCGGCCTGCACGTGGAGTTCACCGTTCTTGATCACCGCGGTGCGGATGGCAATGGCGGTGTCCATGTTGCCGTTCCATGCCAGGTAGCCGACCGCGCCGCCGTAGACTCCACGCTTGACCGGCTCCAGCTCGTCGATGATCTCCATGGCGCGGATCTTCGGCGCGCCGGACAGCGTACCCGCCGGCAGGATCGCCCGCAGCGCGTCCATCGCGCTGAGCCCCTCGCGCAATTGCCCGGTGACGTTGGACACGATGTGCATGACGTTGGAGTAACGTTCGATCACCATTTTTTCGGTGACCTTCACCGCGCCGATATCGGACACCCGCCCCACGTCGTTGCGCCCCAGGTCGATCAGCATCAGGTGCTCGGCGATCTCCTTGGCGTCCGACAGCAGATCCTGCTCCAGCGCCAGGTCGGCCTCTTCGTTGATCCCGCGCGGACGGGTACCGGCGATCGGGCGCACCGTCACCAGGCCATCCTCGACCCGTACCAGCACCTCCGGCGAGCTGCCCACGACATGGAAGTCGCCGAAGTTGAAGAAGTACATGTAGGGCGTCGGATTGAAACAGCGCAGCGCGCGGTACAGGTCGATGGGCGCCGCCTTGAATTCGATGGACATGCGCTGCGACGGCACCACCTGCATGCAGTCGCCGGCCAGGATGTAGTCCTTGATCCTTCCTACCGCGTCTTCATAGTCCTCGCGGGTGAAGCTGGCACGAAACGCCAGCTCACGCCTTGGGGNCCCGCACANAGTGGGGGCGGGTGCCCCATCTGCGTGCCGTGAAGTTCACCGTTATAGATGANCGNTGTACGGATAGTCNTAGCTGTNTCATGTTGCCTTCAATGTCACGTGCAATCGCGCGCGTAGACCNCTCGACGGACGCCTCTCATGATATCCTGGCGGCAGTACGCTGCGCAGCGCANNGNAAGTNGNNNCATGGCTNGCCTCGGCAATGTCGGATCAG Allele: 8 P. aeruginosa IISR6

  22. >acsA-IISR51 CNNCAGGGGTTCTTGANTACCGTCCGGGCGAGTCTACTGGTGCACCGCCGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCACACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCTGCAGCCAAGCCGTTCTTCGGCGTGGTACCGGCGCTGGTGGACAACCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTGGAAAGCGCGATGGTCGCCCACCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGGGCAGCA Allele: 15 >aroE-IISR51 TGNNTTNGNGGACGGATTTTGAGACGTACGTCTCAAATGCTGCCTGACAACTCAGACAGTTGCCAATTGCCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCACGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCGGTCGGTTCCTTGGCATACATCATGTCGTAGCAAACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGACCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGCGCACCGCGCCGAGATCGGCGAACCGCTCGGCCAGATCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAGCTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCGCGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAAGCCCGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCCAGGCGGATGAGGGTGTTCACCGCCCCGGCCCGGGTGGCCCGCTCGCTCAATTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGCTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGGGTGGGACGGGACGAGCGGACTCGTCCCCTGCGGGCGGGTCAGGGCTGGCCGGCGAGCCAATCGCGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTCCTCNNNNCCGGCC Allele: 121 >guaA-IISR51 NGGNNNNNANACCCCANNGACTNNNNGGCCCTGCTGGTGTGGGTGACTCCGGGGGGGAATTTCAGCCGTGGTAGCCGCGGATCTCTGTCTNCNCGANGAGTGTGATGAGCACGGCGACAAGGTCACCGAGATGCCGGCCGGCTTCCACATCCTGGCCAGCACCCCGAGCTGCCCGATCGCCGCCATGGCCGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAGGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGGTACCACAGACAGCCCGGGGGCGTTCAGTTCNATGGCTCAAAGATCTAGTAACGGCTTGCAAATTCNGCCCGGGATANTCACNCCANTACCATGCCNNNAGGCGCATTCC Allele: 36 >mutL-IISR51 NCGAGGTCATGGTNNNCGCGCAACCGAANTATCGAGGCAAGCGACTTCGCCNACGAAGCCGAGGCTTCATCACCCGCTCCATGNCNTCAATAGTCCCCCGCGTGAGTGTCGAGCGTCCCGCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGACGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTANGGGGNCACCNGAA Allele: 5 >nuoD-IISR51 CCCCCGNTNGCCNACGCCACTGGTACGAGCGCGAAGTGTGGGACATGTACGGCATCACCTTCACCGGCCACCCGCACCTGACCCGCATGCTGATGCCGCCGACCTGGCAGGGCCATCCGCTGCGCAAGGACTACCCGGCGCGCGCCACCGAGTTCGATCCCTACTCCCTGTCCGCCGCCAAGCAGGACCTGGAGCAGGAAGCCCTGCGCTTCAAGCCGGAAGACTGGGGCATGAAGCGCCACGGCGAGAACGAGGACTACATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCATCCTGCAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACCGCGGCGCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACCGCATCGACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCTCGCCGGGATCAAGGTGCCCCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTTCCGTATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACAAGGTGGTCGAGGCCATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGCGGCGTCGCCCACGACCTGCCGCGCGGCTGGGACAAGCTGGTCCGCGAGTTCCTCGACTGGATGCCCAAGCGCCTCGACGAGTACGAGACCGCGGCCCTGAAGAACAGCATCTTGCGCGGCCGGACCATCGGCGTCGCCCAGTACAACACCAAGGAAGCCCTCGAGTGGGGCACCACCGGCGCCGGCCTGCGCGCCACCGGTTGCGACTTCGACCTGCGCAAGGCCCGCCCCTACTCCCGGCTACGAGAACTTCGAGTTCGAGGTACCGCTGGCCCACACGGCGACGCTACGACGCTGCNTNNNNNN Allele: 4 >ppsA-IISR51 NCGNCAAAATGNTTCTTGCNTATNNGNNNNTTTTGNGTGCTTCCTTNNGTATGTANGACGTCGATCGTGCCGATCGCGCCCGCTTCGCCTGAGCGACGCCGAGGTCACCGAGCTGGCCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTCCCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCATGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAAGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCACCTGATCGGCGCAAGCTCTACGAGCCGGAGAAAAGAGCAACACCTNCNACCATTGNNNGCANNNCAANN Allele: 15 >trpE-IISR51 GATACCGCCGCCGGCCTGCACGTGGAGTTCACCGTTCTTGATCACCGCGGTGCGGATGGCAATGGCGGTGTCCATGTTGCCGTTCCATGCCAGGTAGCCGACCGCGCCGCCGTAGACTCCACGCTTGACCGGCTCCAGCTCGTCGATGATCTCCATGGCGCGGATCTTCGGCGCGCCGGACAGCGTACCCGCCGGCAGGATCGCCCGCAGCGCGTCCATCGCGCTGAGCCCCTCGCGCAATTGCCCGGTGACGTTGGACACGATGTGCATGACGTTGGAGTAACGTTCGATCACCATTTTTTCGGTGACCTTCACCGCGCCGATATCGGACACCCGCCCCACGTCGTTGCGCCCCAGGTCGATCAGCATCAGGTGCTCGGCGATCTCCTTGGCGTCCGACAGCAGATCCTGCTCCAGCGCCAGGTCGGCCTCTTCGTTGATCCCGCGCGGACGGGTACCGGCGATCGGGCGCACCGTCACCAGGCCATCCTCGACCCGTACCAGCACCTCCGGCGAGCTGCCCACGACATGGAAGTCGCCGAAGTTGAAGAAGTACATGTAGGGCGTCGGATTGAAACAGCGCAGCGCGCGGTACAGGTCGATGGGCGCCGCCTTGAATTCGATGGACATGCGCTGCGACGGCACCACCTGCATGCAGTCGCCGGCCAGGATGTAGTCCTTGATCCTTCCTACCGCGTTTTCATAGTCCTCGCGGGTG Allele:8 P. aeruginosa IISR51

  23. >acsA-IISR13 TGCTATCACTCTNGGGTTTTATNGTCGCTGACCCACGAGCGGGTCTTCGACTACCGTCCGGGCGAAGTCTACTGGTGCACCGCCGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCTGCAGCCAAGCCGTTCTTCGGCGTGGTACCGGCGCTGGTGGACAACCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATAAGCACCGCCGAGGTGGAAAGCGCGATGGTCGCCCACCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGGGCACCCATTCTCTTTAGAGNGAGGGGGGCGTC Allele: 15 >aroE-IISR13 GGNTTNNNGGGACGGATTTGAGACGTACGTCTCAAATGCTGCCTGACAACTCAGACAGTTGCCAATCGCCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCACGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCGGTCGGTTCCTTGGCATACATCATGTCGTAGCAAACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGACCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGCGCACCGCGCCGAGATCGGCGAACCGCTCGGCCAGATCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAGCTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCGCGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAAGCCCGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCCAGGCGGATGAGGGTGTTCAACGCCCCGGCCCGGGTGGCCCGCTCGCTCAATTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGCTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGGGTGGGNAACGGGACGAGCGAACTCGTCCCCTGGCGGGCGGGTCAGGGCTGGCCGGCGAGCCAATCGCGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTCCCCGNNNCGGGCA Allele: 121 >mutL-IISR13 NGNNGCGGTACCAGAGGTCATGTCNGCGCGNACCAACNATCNCGCCAGCGCCTCNCCANNANGCCGAGGCTCATCACCGCTTCTCAGNATCTTNGTCCGCGTGAGCTGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTAAGAGGAGACNCCTNNN Allele: 5 >mutL-IISR13 NGNNGCGGTACCAGAGGTCATGTCNGCGCGNACCAACNATCNCGCCAGCGCCTCNCCANNANGCCGAGGCTCATCACCGCTTCTCAGNATCTTNGTCCGCGTGAGCTGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGTTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGTGACATGCAGCCGCGGGTACAGCCGGCGGCGCACCCGGTGGGGACCAGCGTCGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGCGCCGAGAAGACCGAGTTCGACCATCTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAG CCGGATGGTCCATGACTTCCTCTAAGAGGAGACNCCTNNN Allele: 5 >nuoD-IISR13 NANTGCCCACGCACTGGTACGAGCGCGAAGTGTGGGACATGTACGGCATCACCTTCACCGGCCACCCGCACCTGACCCGCATGCTGATGCCGCCGACCTGGCAGGGCCATCCGCTGCGCAAGGACTACCCGGCGCGCGCCACCGAGTTCGATCCCTACTCCCTGTCCGCCGCCAAGCAGGACCTGGAGCAGGAAGCCCTGCGCTTCAAGCCGGAAGACTGGGGCATGAAGCGCCACGGCGAGAACGAGGACTACATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCATCCTGCAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACCGCGGCGCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACCGCATCGACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCTCGCCGGGATCAAGGTGCCCCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTTCCGTATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACAAGGTGGTCGAGGCCATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGCGGCGTCGCCCACGACCTGCCGCGCGGCTGGGACAAGCTGGTCCGCGAGTTCCTCGACTGGATGCCCAAGCGCCTCGACGAGTACGAGACCGCGGCCCTGAAGAACAGCATCTTGCGCGGCCGGACCATCGGCGTCGCCCAGTACAACACCAAGGAAGCCCTCGAGTGGGGCACCACCGGCGCCGGCCTGCGCGCCACCGGTTGCGACTTCGACCTGCGCAAGGCCCGCCCCTACTCCGGCTACGAGAACTTCGAGTTCGAGGTACCGCTGGCCCACACGGCGACGCTACGACGCTGCANNNNGAAN Allele: 4 >ppsA-IISR13 NTCGAGGGCCATATNCNNTCGATGATCTTGCCTNCTNNGGTCGCNNNNTNNCAGTAGTGACGTCGATCGTGCCGATCGCGCCGCTTCGCCCTGAGCGACGCCGAGGTCACCGAGCTGGCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTTTCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCATGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAAGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCAACCTGATCGGCGCAAGCTCTACGAGCCTGAGNNNNTACCTNNACCATNNCGCAGCNACCANNANNNNTTNGCAGAAAGG ppsA: 15 >trpE-IISR13 GGGGGGNGGNTNTCGCTTACCGCGAGGACTATGAAACGCGGTAGGAAGGATCAAGGACTACATCCTGGCCGGCGACTGCATGCAGGTGGTGCCGTCGCAGCGCATGTCCATCGAATTCAAGGCGGCGCCCATCGACCTGTACCGCGCGCTGCGCTGTTTCAATCCGACGCCCTACATGTACTTCTTCAACTTCGGCGACTTCCATGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGACGGTGCGCCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCGCTGGAGCAGGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTGGGGCGCAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATGGTGATCGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCGAGGGGCTCAGCGCGATGGACGCGCTGCGGGCGATCCTGCCGGCGGGTACGCTGTCCGGCGCGCCGAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTCTACGGCGGCGCGGTCGGCTACCTGGCATGGAACGGCAACATGGACACCGCCATTGCCATCCGCACCGCGGTGATCAAGAACGGTGAACTCCACGTGCAGGCCGGCGGCGGTATCGTTGCCGACTCGGTGCCGGCGCTGGAGTGGGAAGAACCATCAACAGGCGCCGGACCGAGTCGGCACGATCCGCCACAGCTGCAGTGGAGTTCACGGTTTGATCACGGCGTGCGAGGCATGCGTTCATGTGGCTTTCAGCGGTAGCACGCGCCNAAANNCGCTGGACGGCGCGCGNCNTAATTCNGGCGGNAAATTACGCGCCGNNNNGNC Allele: 8 P. aeruginosa IISR13

  24. >acsA-IISR853 TATTCTGGTGTTTCGCCTCGCTGACCCACGAGCGGGTCTTCGACTACCGTCCGGGCGAAGTCTACTGGTGCACCGCCGACATCGGCTGGGTCACCGGCCACACCTACATCGTCTATGGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACCCCGACGTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGCGCCGACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGACGGTTCCAGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGTGGTACTACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAGACCGGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCCGCGGCCAAGCCGTTCTTCGGCGTGGTACCGGCACTGGTGGACAATCTCGGCAACCTGATCGAGGGCGCCGCCGAGGGCAACCTGGTGATCCTCGACTCCTGGCCGGGCCAGGCGCGGACCCTGTTCGGCGACCATGACCGCTTCGTCGACACCTACTTCAAGACCTTCAAGGGCATGTACTTCACCGGCGACGGCGCGCGCCGCGACGAGGACGGCTACTACTGGATCACCGGGCGGGTCGACGACGTGCTCAACGTCTCCGGCCACCGCATGGGCACCGCCGAGGTGGAAAGCGCGATGGTCCGCCCACCCCGAAGGTCGCCGAGGCGGCGGTGGTCGGCATGCAGCACGACATCAAGGAGGGGATTTTTTTTTTNATAAGCCNCCCTGTTAGCCNGGTAACTTNGGTGGGGCACATGGTTCACTNGTCGTCCAGCCGTGGCGGAGACTTCAGAGTCTCACCGCGTGATCTTTNTAGCCNCNCTNCGCGNCNCCGNTCAATCANGTCNNGANGTTTGANNNAGTTTCGACAAAACGCTTAGNATGCCTACAGGGACCNNN Allele: 22 >aroE-IISR853 GGAGAGGGTAAANCCATTTTGAGACGTACGTCTCAAATGCCGCCTGACAACTCAGACAGTTGCCAACTGTCGGCGCAGCGTCTCCAACACTGGCGCCGAGGCAGGACGCACGCCGCGCCAGAGGAAGAATGCCTCGGCGGCCTGCTCCACCAGCATGCCCAGGCCATCCAGGGTACGCGCCGCACCGCGTTCGGCGGCCCAGCGGTTGAAGGCAGTCGGTTCCTTGGCATACATCATGTCGTAGCAGACGGTACGGCCGGGCTCGATCACGCTCTGCGCCAGCGGCGGCACGTCGCCGGCAAGACTGGCCGAGGTGCCGTTGACGATCAGGTCGAAAGGCCCTTCGACCTCGGCGAAACCGCAGCCGTGCACCGCGCCGAGGTCGGCGAACCGCTCGGCCAGGTCCACGGCCTTCCGCGCCGTGCGGTTGGCGATCAGCAACTCCGCCGGGCACTCGCCGAGGAAGGGTTCGAGCACCCCACGCACCGCACCGCCGGCGCCGAGCAGGAGAACCCGCTTGCCGCGCAGCTCGACCCCGGCGTTCGCCGTCAGGTCCCGCAGCAGGCCGGCGCCGTCGGTGTTGTCGCCGCGCAGGCGACCGTCGGCGAGGCGGATCAGGGTGTTCACCGCCCCGGCCCGGGTGGCCCGCTCGCTCAACTCGTCCACCAGACGATAGGCCTCTTCCTTGAACGGCACGGTGACATTGGCGCCCTTGCCCTGCTCGAAGAAGCGCCGGGCGAACCCGGGGAAATCGTCCAGCGGCGCCAGTTGCGCGTCATAGACCAGCGCCTCGCCGGTCTGCTCGGCGAACAGGCGGTGGATCAGCGGCGACTTGCTGTGGCCGATGGGGTTGCCGAATACGCAATAGCGGTCCATGGGTCGTTCCTTTGCCGGAGTGGGGACGGGACGAGCGGACTCGTCCCCTGCGGGCGGGTCAGGGCTGGCCGGCGAGCCAATCGCGGTCGGCGAGGAAATACTCGGTCAGGCGCGCTCCCCNCCCNCGGNN Allele: 5 >guaA-IISR853 NGTAGTGAGNAAACCAAAGAACTGGCCNNCCTGCTGGTGNTGGGTGACTTCCGGGGGAATTCCAGCGGTCGTGGGCGCGGGATTTCTGGCCTTNNAACGTGTGATGAGCCACGGCGACAAGGTCACCGAGATGCCGGCCGGCTTCCACATCCTGGCCAGCACCCCGAGCTGCCCGATCGCCGCCATGGCCGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTAATCGAAGTTTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCAGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAGGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGGTACACAGACAGCCCGGGGCGTTCAGTACCAATGCCAAGNTCTAGCTACGCCNTGCAATTCCGCNNCGTAGTCNCTCCTACACCNGCNNGNNTGCCCANTCCTTGNNNCT Allele: 83 >mutL-IISRT853 AAGTCATGGTCAGGCGGCGCAACCNGGTTATCGAGNCAAGCCGCCTCGCCACGGAAGCGGGGGCTCATCACCCGCTCCTCGTANCATCGTCCGCCGGTGAGGTGGTCGAGCGTCCCGCCTCGGTGGCCAAGGAACTCTGGAAAACAGCCTTGACGCCGGTTCCCGGCGCATTGATGTGGAGGTCGAGCAGGGCGGCATCAAGTTGCTGCGAGTGCGCGACGACGGTCGCGGCATCCCCGCCGACGACCTGCCGCTGGCCCTGGCTCGCCACGCCACCAGCAAGATCCGCGAGCTGGAAGACCTGGAGCGGGTGATGAGCCTCGGCTTCCGTGGCGAGGCGCTGGCCTCGATCAGCTCGGTAGCGCGCCTGACCATGACCTCGCGTACCGCCGACGCCGGCGAAGCCTGGCAGGTGGAAACCGAGGGCCGCGACATGCAGCCGCGGGTACAGCCGGCGGCGCATCCGGTGGGGACCAGCGACGAGGTTCGCGACCTGTTCTTCAACACCCCGGCGCGGCGCAAGTTCCTGCGTGCCGAGAAGACCGAGTTCGACCATCTGCAGGAGGTCATCAAGCGCCTGGCGCTGGCCCGCTTCGACGTGGCTTTCCACCTGCGCCACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGCGCGCGCCGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAGGTCGAGCGCAACGGTCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCCAGCCGGACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCCACGCGGTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCTGTTCTTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAGTTCGCTTCCGTGACAGCCGGATGGTCCATGACTTCCTCTATGGGAACCCTGA Allele: 2 >nuoD-IISR853 TNNAGCAGCGGTCGTAGGCGTCGCCGTTGTGGGCCAGCGGTACCTCGAACTCGAAGTTCTCGTAGCCGGAGTAGGGGCGGGCCTTGCGCAGGTCGAAGTCGCAACCGGTGGCGCGCAGGCCGGCGCCGGTGGTGCCCCACTCGAGGGCTTCCTTGGTGTTGTACTGGGCGACGCCGATGGTCCGGCCGCGCAGGATGCTGTTCTTCAGGGCCGCGGTCTCGTACTCGTCGAGGCGCTTGGGCATCCAGTCGAGGAACTCGCGGACCAGCTTGTCCCAGCCGCGCGGCAGGTCGTGGGCGACGCCGCCGATGCGGTACCAGGCCGGGTGCAGGCGGAAGCCGGTGATGGCCTCGACCACCTTGTAGGCGCGCTGGCGGTCGGGGAAGGTGAAGAACACCGGGGTCATGGCGCCGACGTCCTGGATATAGGTGCCCAGGTACAGCAGGTGGTTCAGGATACGGAAGAACTCCGCCATCATGATCCGGATCACGTCGACCCGCTGGGGCACCTTGATCCCGGCGAGCTTCTCCACCGAGAGTACGTAGGGCAGGTTGTTCATCACCCCGCCGAGGTAGTCGATGCGGTCGGTGTAGGGGATGAAACTGTGCCAGGACTGGCGCTCGGCCATCTTCTCGGCGCCGCGGTGGTGGTAGCCGATCTCCGGGACGCAGTCGATGATCTCCTCGCCGTCCAGTTGCAGGATGATGCGGAACGCGCCGTGGGCGGACGGGTGGTTCGGGCCGAGGTTGAGGAACATGTAGTCCTCGTTCTCGCCGTGGCGCTTCATGCCCCAGTCTTCCGGCTTGAAGCGCAGGGCTTCCTGCTCCAGGTCCTGCTTGGCGGCGGACAGGGAGTAGGGATCGAACTCGGTGGCGCGCGCCGGGTAGTCCTTGCGCAGCGGATGGCCCTGCCAGGTCGGCGGCATCAGCATGCGGGTCAGGTGCGGGTGGCCGGTGAAGGTGATGCCGTACATGTCCCACACTTCGCGCTCGTACAGTGGCGTGNCNNNNGGG Allele: 4 >ppsA-IISR853 CTATCGGGGCANTAGNNNNTTGGGCNGTTTTTNNNNGNCNNGTTGNTCTNCGCTNNAGGTAGTGACGTCGATCGCGCCGATCGCGCCCGCTTCGCCTGAGCGACGCCGAGGTCACCGAGCTGGCCAAGCAGGCCATGATCATCGAGAAGCACTATGGCCGCCCGATGGACATCGAATGGGCCAAGGACGGTGACGACGGCAAGCTGTACATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATGGAGCGCTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCAGCGCATCGGTGCCGGTCCGGTCAAAGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAACCGGGTGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGCGCCAGCGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGCGAACTGGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGCCAGGGGGTGACCGTTTCCTGTGCCGAAGGCGATACCGGCTTCATCTTCGAAGGCGAACTCGGTTTCGATGTGCGCAAGAACTCGGTCGACGCCATGCCCGACCTTCCGTTCAAGATCATGATGAACGTCGGCAATCCCGATCGCGCTTTCGATTTCGCCCAGTTGCCGAACGAAGGCGTGGGCCTGGCCCGCCTCGAATTCATCATCAACCGCAAGATCGGCGTGCATCCCAAGGCGTTGCTGAACTTCGCCGGCCTGCCGGCGGACATCAAGGAAAGCGTGGAGAAGCGCATCGCCGGCTATCCCGATCCGGTCGGCTTCTACGTCGAGAAGCTGGTGGAGGGCATCAGCACCCTGGCCGCGGCGTTCTGGCCGAAGAAGGTCATCGTGCGCCTGTCCGACTTCAAGTCCAATGAGTACGCCAACCTGATCGGCGCAGCTCTACACNNGNNGCCCNNACCNGCCCCNCTACTNCACNCAA Allele: 13 >trpE-IISR853 CTCACTTGTCGATATGTCCATGGCGGGATTTCGCGCGCGGAAGAGTGCCCGCTGGAAGATCGCCCGCAGCGGCGTCCACCGCGTTGAGCCCCCCGCGCAATTGCCCGGTGGACGGTCGGGCCCGATGTGCACGGCGTTGGGAGGTAACCTTCCATCANCCATTTTTTTCGTGTGACCTTTCACCGCGCCGATATCGGACACCCGCCCCACGTTCGCGGGGGCCCCCAAGGGCGTGAGCGGCGTTTCGTGCCAGCTTCACCCGCGAGGACTATGAAAACGCGGTAGGAAGGATCAAGGACTACATCCTGGCCGGCGACTGCATGCAGGTGGTGCCGTCGCAGCGCATGTCCATCGAATTCAAGGCGGCGCCCATCGACCTGTACCGCGCGCTGCGCTGTTTCAATCCGACGCCCTACATGTACTTCTTCAACTTCGGCGACTTCCATGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGACGGTGCGCCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCACTGGAGCAGGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTGGGGCGCAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATGGTGATCGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCGAGGGGCTCAGCGCGATGGACGCGCTGCGGGCGATTCTGCCGGCGGGCACTCTATCCGGCGCGCCGAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTCTACGGCGGCGCGGTCGGCTACCTGGCATGGAACGGCAACATGGACACCGCCATTGCCATCCGCACCGCGGTGATCAAGAACGGTGAACTCCACGTGCAGGCCGGCGGCGGTATCGTTGCCGACTCGGTGCCCGCGCTGGAGTGGGAAGAAACCATCACACCCCCCGGGACCAAATCGGCACGATTCCGCCTCGCTGCAGTGAGTTAACCGTTTGGATGACGCGTTCGGAAGGCATGGCGGTTTCATGTGCNTTCAGCNGGTNCNNCGNNNCTAAACCCGCTGGACGGNNNNNTCATNTATTCGGGGCGAANTNGGCGCNAAGAGGGCNGANAAATGCCGCNGCTTGCCNTGNNGCTCANNTGNACGAATTNNNACAAATTGANN Allele: 7 P. aeruginosa IISR853

  25. >acsA-GEB9 NTTGTNCAAAAGAATTGCTGCCCCTTGATGGTCGTGCTGCATGCCGACCACCGCCGCCTCGGCGACCTTCGGGTGGGCGACCATCGCGCTTTCCACCTCGGCGGTGCCCATGCGGTGGCCGGAGACGTTGAGCACGTCGTCGACCCGCCCGGTGATCCAGTAGTAGCCGTCCTCGTCGCGGCGCGCGCCGTCGCCGGTGAAGTACATGCCCTTGAAGGTCTTGAAGTAGGTGTCGACGAAGCGGTCATGGTCGCCGAACAGGGTCCGCGCCTGGCCCGGCCAGGAGTCGAGGATCACCAGGTTGCCCTCGGCGGCGCCCTCGATCAGGTTGCCGAGATTGTCCACCAGTGCCGGTACCACGCCGAAGAACGGCTTGGCCGCGGAGCCCGGCTTCATCGCGTGGGCGCCCGGCAGCGGGGTCATCAGGCAGGCGCCGGTCTCGGTCTGCCACCAGGTGTCGACGATCGGGCAGCGCGACTGGCCGACGGTCTCGTAGTACCACTGCCAGGCTTCCGGGTTGATCGGCTCGCCCACCGAACCGAGCAGACGCAGGCTGGAACCGTCGGCACCGGCCACCGCCGCCTTGCCTTCGGCCATCATCGCGCGGATCGCGGTCGGCGCGGTGTAGAGGATGTTGACCTTGTGCTTGTCGATGATTTTCGCCACGCGGGTCACGTCGGGGTAGTTCGGCACGCCCTCGAACAGAATGGTGGTGGCGCCGTTGGCCAGCGGGCCATAGACGATGTAGGTGTGGCCGGTGACCCAGCCGATGTCGGCGGTGCACCAGTAGACTTCGCCCGGACGGTAGTCGAAGACCCGCTCGTGGGTCAGCGAGGTAANANCCNGAGATCGA Allele: 22 >aroE-GEB9 AGCCGGGCAGAGAGGAAGGCGCCTGACCGAGTATTTCCTCGCCGACCGCGATTGACTCGCCGGCCAGCCCTGACCAGCCCGCAGGGGACGAGTCCGCTCGTCCCGTCCCACTCCGGCAAAGGAACGACCCATGGACCGCTATTGCGTATTCGGCAACCCCATCGGCCACAGCAAGTCGCCGCTGATCCACCGCCTGTTCGCCGAGCAGACCGGCGAGGCGCTGGTCTATGACGCGCAACTGGCGCCGCTGGACGATTTCCCCGGGTTCGCCCGGCGCTTCTTCGAGCAGGGCAAGGGCGCCAATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGACGAGTTGAGCGAGCGGGCCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTCGCCGACGGTCGCCTGCGCGGCGACAACACCGACGGCGCCGGCCTGCTGCGGGACCTGACGGCGAACGCCGGGGTCGAGCTGCGCGGCAAGCGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGTGGGGTGCTCGAACCCTTCCTCGGCGAGTGCCCGGCGGAGTTGCTGATCGCCAACCGCACGGCGCGGAAGGCCGTGGACCTGGCCGAGCGGTTCGCCGACCTCGGCGCGGTGCACGGCTGCGGTTTCGCCGAGGTCGAAGGGCCTTTCGACCTGATCGTCAACGGCACCTCGGCCAGTCTTGCCGGCGACGTGCCGCCGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTATGCCAAGGAACCGACTGCCTTCAACCGCTGGGCCGCCGAACGCGGTGCGGCGCGTACCCTGGATGGCCTGGGCATGCTGGTGGAGCAGGCCGCCGAGGCATTCTTCCTCTGGCGCGGCGTGCGTCCTGCCTCGGCGCCAGTGTTGGAGACGCTGCGCCGACAGTTGGCAACTGTCTGAGTTGTCAGGCGGCATTTGAGACGTANGTNNCAATTTGGNTTCCATCTG Allele: 5 >guaA-GEB9 GNGAGCGCGAGAGAATNGCGCNNNCCTTCCTTGGTNGGGNTNNATCTCGGGGGGGGAATTGCNNGCCGTCGTAGGCGCGGGATCTGTCGGTCTCTTAAGCGGTGTGATGAGCCACGGCGACAAGGTCACCGAGATGCCGGCCGCGCTTCCACAGTCCGTGGCCAGCAGCCCCGAGCTGCCCGATCGCCGCCATGGCCGACGATGCCCGCGCCTACTACGGCGTGCAATTCCACCCGGAAGTCACCCACACCAAGCAGGGCCTGCGCATTCTCTCGCGCTTCGTCCTCGACATCTGCGGTTGCGCCGCGCTGTGGACCCCCTCGAACATCGTCGACGACGCCATCGCCACCGTGCGCGCCCAGGTCGGTTCCTCCAAGGTCCTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACAAGGCCATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACGAAGGCGACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCAACGCCGAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGCAAGATCATCGGCCGCACCTTCATCGAAGTTTTCGACGAAGAAGCCACCAAGCTGCAGGACGTGAAGTTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCAGCCGGCGCCAAGACCGGCAAGGCCCACGTGATCAAGTCGCACCACAACGTCGGCGGCCTGCCGGAGGACATGCAGTTCGAACTGGTCGAGCCGCTGCGCGAACTGTTCAAGGACGAAGTGCGCAAGATCGGCCTGGAGCTGGGCCTGCCCTACGACATGGTCTACCGCCACCCGTTCCCCGGCCCGGGCCTGGGCGTGCGCATCCTCGGCGAGGTGAAGAAGGAGTACGCCGACCTGCTGCGCCAGGCCGACCACATCTTCATCGAGGAACTGCGCGCCTTCGACTGGTACCACAAGACACCCGGGGCGTATCAGCTCCNCGATCACAACTTGTNCGGCTGGCAATTCACGCCGGAATTTCCTCATCAACTCGGGGAAGANCCNNNCCTGGTCCN Allele: 83 >mutL-GEB9 TCAGGTGNTCCCGTAGAGGGAAGGTCATGGACCATCCGGCTGTCACGGAAGCGAACTTCGTGCCTGGTCGGGTGCACGTTGACGTCCACCACCGCCGGATCGACTTCGAAGAACAGCACGAAGGTCGGATGCCGGCCGTTGTACAGCACGTCGCGATAAGCCTGGCGCACCGCGTGGGCGACCAGCTTGTCGCGCACCATGCGCCCGTTCACATAGAAGTACTGCAGGTCCGGCTGGCTGCGGGAGAAGGTCGGCAAGCCGACCCAGCCCCACAGGTGCAGACCGTTGCGCTCGACCTCGATCGGCAGCGCCTGCTCGAGGAATGCCTGGCCGCACACCGCGCCGACCCGGCGCGCGCGGGCCAGCTCGTCTCGCGCCTCGTGCAGGGCGAAGATGGTCTTGCCGTTGTGGCGCAGGTGGAAAGCCACGTCGAAGCGGGCCAGCGCCAGGCGCTTGATGACCTCCTGCAGATGGTCGAACTCGGTCTTCTCGGCACGCAGGAACTTGCGCCGCGCCGGGGTGTTGAAGAACAGGTCGCGAACCTCGACGCTGGTCCCCACCGGATGCGCCGCCGGCTGTACCCGCGGCTGCATGTCGCGGCCCTCGGTTTCCACCTGCCAGGCTTCGCCGGCGTCGGCGGTACGCGAGGTCATGGTCAGGCGCGCTACCGAGCTGATCGAGGCCAGCGCCTCGCCACGGAAGCCGAGGCTCATCACCCGCTCCAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGGCGAGCCAGGGCCAGCGGCAGGTCGTCGGCGGGGATGCCGCGACCGTCGTCGCGCACTCGCAGCAACTTGATGCCGCCCTGCTCGACCTCCACATCAATGCGCCGGGAACCGGCGTCAAGGCTGTTTTTCCAGGAGTTCCTTGGCCACCGAGGCGGGACGCTCGACCACCTCACCGGCGGGGACATCATGAGATTGGAGCGGGTGATGANCCTTGGCTTCCNGGGAGAGGCCGCTCGCCTCCGATCATCTNTTTGTNCGTCTGACCATGACCTCTCGCN Allele: 2 >trpE-GEB9 ANNNTGATCGATTAANTTCCNNGNGGGATGTTCGGCGCGCAGGAAAGAGTGCCCGCCTGCAGAATTCGCCCGCAGACGCNTCCACGCGATTGAGCCCCACGCGCAATGACCCGGTGACCGTGGGCCCGATGTGCACGACGTTGGAGTAACGTTCGATCACCCATTTTTTCGGTGACCTTCACCGCGCCGAAATNGGACACCCCGCCCCACGTCGTCTGCGGCCCCCAGTCGTGAGCCGGCGTTTCGTGCCAGCTTCACCCGCGAGGACTATGAAAACGCGGTAGGAAGGATCAAGGACTACATCCTGGCCGGCGACTGCATGCAGGTGGTGCCGTCGCAGCGCATGTCCATCGAATTCAAGGCGGCGCCCATCGACCTGTACCGCGCGCTGCGCTGTTTCAATCCGACGCCCTACATGTACTTCTTCAACTTCGGCGACTTCCATGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGACGGTGCGCCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCACTGGAGCAGGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTGGGGCGCAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATGGTGATCGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCGAGGGGCTCAGCGCGATGGACGCGCTGCGGGCGATTCTGCCGGCGGGCACTCTATCCGGCGCGCCGAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTCTACGGCGGCGCGGTCGGCTACCTGGCATGGAACGGCAACATGGACACCGCCATTGCCATCCGCACCGCGGTGATCAAGAACGGTGAACTCCACGTGCAGGCCGGCGGCGGTATCGTTGCCGACTCGGTGCCCGCGCTGGAGTGGGAAGAAACCATCACAGCCCGCCGGGACCCGAGTCGCACGATACCGCCCCCCGGCCTGCAGTGGAAGTTCACCTTTTTGATCACCGCGATGCGGAAGCAATTGGCNNNTTCCACGTTTGNCTTTCANGNCAGTAGGCCACAGCGCCCCCTTAGAACCAGCTNGACCGCTCCNNCTTNATATAATNTCTGGGNGANATTTNCGCCGCTGANGAGTGCTCTGAAGAATCCTCATNNGACATCCGC Allele: 7 >nuoD-GEB9 TCTGACATGCAGCGGTCGTAGGCGTCGCCGTGTGGGCCAGCGGTACCTCGAACTCGAAGTTCTCGTAGCCGGAGTAGGGGCGGGCCTTGCGCAGGTCGAAGTCGCAACCGGTGGCGCGCAGGCCGGCGCCGGTGGTGCCCCACTCGAGGGCTTCCTTGGTGTTGTACTGGGCGACGCCGATGGTCCGGCCGCGCAGGATGCTGTTCTTCAGGGCCGCGGTCTCGTACTCGTCGAGGCGCTTGGGCATCCAGTCGAGGAACTCGCGGACCAGCTTGTCCCAGCCGCGCGGCAGGTCGTGGGCGACGCCGCCGATGCGGTACCAGGCCGGGTGCAGGCGGAAGCCGGTGATGGCCTCGACCACCTTGTAGGCGCGCTGGCGGTCGGTGAAGGTGAAGAACACCGGGGTCATGGCGCCGACGTCCTGGATATAGGTGCCCAGGTACAGCAGGTGGTTCAGGATACGGAAGAACTCCGCCATCATGATCCGGATCACGTCGACCCGCTGGGGCACCTTGATCCCGGCGAGCTTCTCCACCGAGAGTACGTAGGGCAGGTTGTTCATCACCCCGCCGAGGTAGTCGATGCGGTCGGTGTAGGGGATGAAACTGTGCCAGGACTGGCGCTCGGCCATCTTCTCGGCGCCGCGGTGGTGGTAGCCGATCTCCGGGACGCAGTCGATGATCTCCTCGCCGTCCAGTTGCAGGATGATGCGGAACGCGCCGTGGGCGGACGGGTGGTTCGGGCCGAGGTTGAGGAACATGTAGTCCTCGTTCTCGCCGTGGCGCTTCATGCCCCAGTCTTCCGGCTTGAAGCGCAGGGCTTCCTGCTCCAGGTCCTGCTTGGCGGCGGACAGGGAGTAGGGATCGAACTCGGTGGCGCGCGCCGGGTAGTCCTTGCGCAGCGGATGGCCCTGCCAGGTCGGCGGCATCAGCATGCGGGTCAGGTGCGGGTGGCCGGTGAAGGTGATGCCGTACATGTCCCACACTTCGNNCTCGTACCAGTCGCGTNGGNCAGNN Allele: 4 >ppsA-GEB9 GGACAGGCGCACGATGACCTTCTTCGGCCAGAACGCCGCGGCCAGGGTGCTGATGCCCTCCACCAGCTTCTCGACGTAGAAGCCGACCGGATCGGGATAGCCGGCGATGCGCTTCTCCACGCTTTCCTTGATGTCCGCCGGCAGGCCGGCGAAGTTCAGCAACGCCTTGGGATGCACGCCGATCATGCGGTTGATGATGAATTCGAGGCGGGCCAGGCCCACGCCTTCGTTCGGCAACTGGGCGAAATCGAAAGCGCGATCGGGATTGCCGACGTTCATCATGATCTTGAACGGAAGGTCGGGCATGGCGTCGACCGAGTTCTTGCGCACATCGAAACCGAGTTCGCCTTCGAAGATGAAGCCGGTATCGCCTTCGGCACAGGAAACGGTCACCCCCTGGCCATCCTGCAGGATCTGGGTGGCGTTGCCGCAACCGACCACCGCCGGGATGCCCAGTTCGCGAGCGATGATCGCCGCGTGGCAGGTGCGCCCGCCGCGGTTGGTGACGATGGCGCTGGCGCGCTTCATCACTGGCTCCCAGTCCGGGTCGGTCATGTCGGAGACCAGGACGTCACCCGGTTGGACCTTGTCCATTTCCGACACGTCGTTGATCACTTTGACCGGACCGGCACCGATGCGCTGGCCGATGGCACGCCCTTCCACCAGGACGGTCCCCTTCTCTTTCAGCAGGTAGCGCTCCATGACCGTGGCGCTGGCGCGGCTCTTCACGGTTTCCGGGCGTGCCTGGACGATGTACAGCTTGCCGTCGTCACCGTCCTTGGCCCATTCGATGTCCATCGGGCGGCCATAGTGCTTCTCGATGATCATGGCCTGCTTGGC CAG Allele: 13 P. aeruginosa GEB9

  26. Allelic profile of P. aeruginosa based on sequence comparison of seven house keeping genes

  27. Population snapshot of relationship among the global P. aeruginosa-burst diagram Created by eBurst programme which is based on a novel eBurst algorithm IISR6 & others BP35 & others

  28. BP35 Dutch Chinese Strain and IISR51, IISR13 & IISR6 Population snapshot of relationship among the global P. aeruginosa-burst diagram Created by eBurst programme which is based on a novel profuse algorithm

  29. 1. MLST analysis revealed that the isolates can be grouped into two IISR6, IISR51 and IISR13 belong to one Sequence Type BP35, IISR853 and GEB9 belong to another Sequence Type iii. PAO1 formed singleton in the population

  30. 2. Comparison of the allelic profile with global collection (932 isolates) revealed that the IISR6, IIASR51 and IISR13 clustered with a Chinese strain (ST760) has origin in human sputum ( submitted by Yun-Song Yu Zhejiang University, PR-China, 02-06-2009)? ii. BP35, IISR853 and GEB9 are SLV of a Dutch strain (ST 575) with unknown history (Submitted by R Van MansfieldUniversity Medical Center Utrecht, Netherlands, 14-03-2008)?

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