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20 o C PowerPoint Presentation

20 o C

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20 o C

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  1. Supplementary Table S1. Respiration rates of the mitochondria isolated from the florets of thermogenic male part of the spadices of D. vulgaris. O2 uptake (nmol O2/min/mg protein) Respiration 20oC 35oC • State 2 38.2±19.1 78.4±50.8** • State 3 31.7±21.6 121.4±23.3** The rates of oxygen uptake were measured using an oxygen electrode cuvette after sequential addition of succinate (0.5 mM; state 2 respiration) and ADP (0.5 mM ADP; state 3 respiration) at either 20oCor 35oC. The data are expressed as the mean ± standard deviation (n = 3). Significant differences between the temperatures were evaluated by Student’s t-test and are shown as asterisks (**P < 0.01).

  2. Supplementary Table S2. Alternative and cytochrome respiration capacity of the mitochondria isolated from the florets of thermogenic male part of the spadices of D. vulgaris. O2 uptake (nmol O2/min/mg protein) Respiration 20oC 35oC 36.1±6.8 • 92.2±11.9** (-Pyr) AOX capacity 48.4±6.4 • 299.0±61.3** (+Pyr) COX capacity • 24.6±8.0 • 55.9±1.6** The rates of oxygen uptake by purified mitochondria were measured using an oxygen electrode cuvette at either 20oCor 35oCin the presence of 100 mM n-propyl gallate or 0.5 mM KCN for measurement of the alternative or cytochrome pathways, respectively. The capacity of the alternative oxidase (AOX) and cytochrome (COX) pathways was measured as described in the Materials and Methods. The results shown represent the mean ± standard deviation of three measurements using the mitochondria from the same preparation. Significant differences between the temperatures were evaluated by Student’s t-test and are shown as asterisks (**P < 0.01).

  3. Supplementary Table S3. DvAOX-specific amino acid sequence (DIESGVIQDSPAPAIALDYWR) detected by nano LC–MS/MS. Bold red italic represents the series that contributed to the score. Bold red indicates that the number of matches in the ion series is greater than would be expected by chance, indicating that the ion series is present. Non-bold red shows that the number of matches in the ion series is no greater than would be expected by chance.