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Characterization of NF-κB p65 Variants: D94E and D97E in Cytosolic Signaling Pathways

This study explores the functional implications of NF-κB p65 variants, particularly D94E and D97E, in cellular signaling. Utilizing pCMV constructs tagged with FLAG and other markers, we analyze the cytosolic localization and interactions of these variants with IκBα and p50/NF-κB1. Our findings suggest that mutations in the p65 subunit influence its regulatory roles in immune responses and inflammation. This research contributes to a deeper understanding of NF-κB signaling mechanisms and their perturbations in various diseases.

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Characterization of NF-κB p65 Variants: D94E and D97E in Cytosolic Signaling Pathways

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  1. (a) (b) (c) 94DCRD97 p65wt 19 551 ATG NH2p65 pCMV-NH2p65-tag pCMV-NH2p65-tag pCMV-p65 D94E;D97E-tag pCMV-p65 D94E;D97E-tag pCMV-p65wt-tag pCMV-p65wt-tag 97 pCMV-p65wt pCMV-p65wt 75 75 p65wt-tag p65wt-tag p65wt NH2p65-tag NH2p65-tag 50 50 IB: āp65 COOH IB: āFLAG Cytosol Cytosol (d) pCMV-p65 D94E;D97E-tag pCMV-p65wt-tag pCMV- NH2p65-tag 75 p65wt-tag IP: āFLAG IB: āp65 COOH, āp50 and āIB p65wt NH2p65-tag 50 p50/NFB1 IB 37 Cytosol

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