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Procedure for plasmid purification PowerPoint Presentation
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Procedure for plasmid purification

Procedure for plasmid purification

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Procedure for plasmid purification

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  1. Procedure for plasmid purification 96 x 1–1.25 ml E. coli bacterial culture in 96-Deepwell Culture Plate Centrifuge Deepwell Culture Plate. Pour off or aspirate supernatants Add Resuspension buffer. Add Lysis buffer. Add Neutralization buffer Pipette each lysate in the Culture Plate to Filter Plate A. Apply vacuum. Add DNA Binding Buffer to Filter Plate DB (on top of Manifold). Apply vacuum . Add Washing buffer to Filter Plate DB. Apply vacuum. Add Molecular Biology Grade Water to DNA binding matrix in Filter Plate DB. Incubate. Gradually apply vacuum to elute plasmid DNA into Collection Plate Result: purified plasmid DNA in Collection Plate Place Filter Plate A on top of Filter Plate DB Place Filter Plate DB on top of Collection Plate