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Gel electrophoresis

Gel electrophoresis. L Mathias. Definition. Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied. . DNA negatively charged. DNA cut up using restriction endonucleases. Make up the gel which the DNA will be put into.

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Gel electrophoresis

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  1. Gel electrophoresis L Mathias

  2. Definition • Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.

  3. DNA negatively charged

  4. DNA cut up using restriction endonucleases

  5. Make up the gel which the DNA will be put into

  6. Dye added to the DNA

  7. Buffer solution added to the tank

  8. DNA samples loaded into wells

  9. Electrical current applied to the chamber

  10. DNA is stained using ethidium bromide

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