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Import sequences of targeted phylum from 16S rRNA ribosomal databases. Extract the N most representative sequences of the phylum. Input the N sequences to the motif search program MEME Identification of n patterns.

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  1. Import sequences of targeted phylum from 16S rRNA ribosomal databases Extract the N most representative sequences of the phylum Input the N sequences to the motif search program MEME Identification of n patterns Test each of the n patterns against the 16S rDNA sequences of the databases -Maximum Hits in the phylum (Sensitivity) -Minimum Hits in the rest of Bacteria Domain (Specificity) Select pattern as a potential barcode is dependent on sensitivity and specificity thresholds NO Patterns are over ? YES Generate the DNA barcode Best scores for sensitivity and specificity

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