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Cell-Cell Communication, Sensing and Chemotaxis

This study delves into the intricate mechanisms of cell-cell communication and chemotaxis, focusing on the interaction between cuttlefish and the marine bacterium Vibrio harveyi. We investigate autoinducers such as AI-1 and AI-2, which enable communication among bacterial cells, and their role in chemotaxis. The research highlights the fascinating natural context of predation and camouflage, revealing how microorganisms coordinate behavior through signaling cascades. Utilizing models from the iGEM competition, we explore the implications of these systems for synthetic biology and biotechnology.

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Cell-Cell Communication, Sensing and Chemotaxis

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  1. Cell-Cell Communication, Sensing and Chemotaxis N Kuldell for 20.020 Spring 2009

  2. Heidelburg iGEM 2008: ecolicense to kill Part 1: Sensing Background information

  3. Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Cuttlefish: master of camouflage During the day they lie buried in the bottom of the ocean; at night they swim and hunt for food.

  4. AI-2 AI-1 Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Few cells: Autoinducers diffuse away More cells: Autoinducers initiate signaling, transcription Photo from B. Bassler, Fig from: Genes & Dev. 2001. 15: 1468-1480 doi: 10.1101/gad.899601

  5. Cell-Cell Communication iGEM context: “prey” pathogen, “predator” killer Prey Cell LuxS= enzyme that produces autoinducer 2 (AI2)…diffuses through the media Predator Cell Nature (2002) 415: 545 LuxP, LuxQ = receptor that binds (AI2)…induces chemotaxis….how?

  6. AI-2 AI-1 Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor histidine kinase “RR” response regulator

  7. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor kinase “RR” response regulator Plant Physiol. (1998) 117: 723

  8. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output Tar Che flagella P CCW, default, tumble CW, signal, swim TCS “SHK” sensor kinase “RR” response regulator J Bacteriol. (2000)182: 6865

  9. Sensing and Signaling Cascades iGEM context: chimeric receptor

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