1 / 9

Cell-Cell Communication, Sensing and Chemotaxis

Cell-Cell Communication, Sensing and Chemotaxis. N Kuldell for 20.020 Spring 2009. Heidelburg iGEM 2008: ecolicense to kill. Part 1: Sensing. Background information. Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi. Cuttlefish: master of camouflage.

jbonaparte
Télécharger la présentation

Cell-Cell Communication, Sensing and Chemotaxis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cell-Cell Communication, Sensing and Chemotaxis N Kuldell for 20.020 Spring 2009

  2. Heidelburg iGEM 2008: ecolicense to kill Part 1: Sensing Background information

  3. Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Cuttlefish: master of camouflage During the day they lie buried in the bottom of the ocean; at night they swim and hunt for food.

  4. AI-2 AI-1 Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Few cells: Autoinducers diffuse away More cells: Autoinducers initiate signaling, transcription Photo from B. Bassler, Fig from: Genes & Dev. 2001. 15: 1468-1480 doi: 10.1101/gad.899601

  5. Cell-Cell Communication iGEM context: “prey” pathogen, “predator” killer Prey Cell LuxS= enzyme that produces autoinducer 2 (AI2)…diffuses through the media Predator Cell Nature (2002) 415: 545 LuxP, LuxQ = receptor that binds (AI2)…induces chemotaxis….how?

  6. AI-2 AI-1 Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor histidine kinase “RR” response regulator

  7. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor kinase “RR” response regulator Plant Physiol. (1998) 117: 723

  8. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output Tar Che flagella P CCW, default, tumble CW, signal, swim TCS “SHK” sensor kinase “RR” response regulator J Bacteriol. (2000)182: 6865

  9. Sensing and Signaling Cascades iGEM context: chimeric receptor

More Related