1 / 22

Genetics of Bacteria

Genetics of Bacteria. Bacterial genome =. Genetics of Bacteria. Bacterial genome = One circular DNA molecule E. coli chromosome has 100 times more DNA than in a typical virus, but much less than a eukaryotic cell. Packed into nucleoid region of cell Plasmid =. Genetics of Bacteria.

calida
Télécharger la présentation

Genetics of Bacteria

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Genetics of Bacteria • Bacterial genome =

  2. Genetics of Bacteria • Bacterial genome = One circular DNA molecule • E. coli chromosome has 100 times more DNA than in a typical virus, but much less than a eukaryotic cell. • Packed into nucleoid region of cell • Plasmid =

  3. Genetics of Bacteria • Bacterial genome = One circular DNA molecule • E. coli chromosome has 100 times more Dna than in a typical virus, but much less than a eukaryotic cell. • Packed into nucleoid region of cell • Plasmid = small circular extra piece of DNA

  4. Bacterial Genetic Recombination • What is the main source of genetic recombination in bacteria? • Mutations • What are the other sources of recombination?

  5. Transformation

  6. Transduction What is the vector of transduction?

  7. Conjugation

  8. Plasmids • What is a plasmid? • Small circular, self replicating piece of bacterial DNA • Plasmid genes are advantageous to the bacteria that has them • Plasmids that confer resistance to antibiotics are called R plasmids

  9. Regulation of Gene expression in prokaryotes (operons) Why is it important that regulation of protein production occur in an organism?

  10. Repressible operons • Repressible operons have structural genes that code for the production of the substrate (anabolic process). • The repressor protein is produced in an inactive form, leaving the operator open • In the presence of the substrate, the substrate will allosterically bind to the repressor protein (is a co-repressor) and activate the repressor protein causing it to bind to the operator

  11. Inducible operons • Inducible operons have structural genes that produce enzymes that break down a substrate (catabolic process). • The repressor is translated into its active configuration and will bind to the operator in the absence of the substrate. • If the substrate is present, it binds to the repressor protein and de-activates it, thereby opening up the operator.

  12. In the lac operon, cAMP acts as a volume control… • What would happen if: • High glucose, high lactose? • Low glucose, high lactose? • High glucose, low lactose? • Low glucose, low lactose?

  13. Each plate…. What amount of growth do you expect? What is the purpose of the plate (hint: what would be indicated if amount of growth did NOT match expected?)? LB plate (-pGLO) LB/amp plate (-pGLO) LB/amp plate (+pGLO) LB/amp/ara plate (+pGLO) Transformation Lab

  14. Gene for regulatory protein for arabinose operon Modified arabinose operon – genes for enzymes replaced with pGLO gene (codes for glowing fluorescent protein = GFP) Ampicillin resistance gene

More Related