1. Liquid Nitrogen and Cryogenics Training Program Prepared by: Environmental Health and Safety

2. Potential HazardsHealth: • Vapors may cause dizziness or asphyxiation without warning. • Vapors from liquefied gas are initially more dense than air and spread along ground. • Contact with gas or liquefied gas may cause burns, severe injury, and/or frostbite.

3. Public Safety • Immediately call emergency personnel in the event of a large spill or leak. 9-1-1 from campus phone or 438-8631 for ISUPD dispatcher • Isolate large spills or leaks for at least 100 meters in all directions. • Keep unauthorized personnel away. • Stay upwind. • Keep out of low areas. • Ventilate closed spaces before entering.

4. Container Requirements • Closed container with a loose fitting top that allows venting. • Use only vessels approved to contain liquid nitrogen. • Vessel must have carrying handles or be on wheels.

5. Potential HazardsExplosion: • Liquid Nitrogen must be transported and stored in an appropriate container containing pressure relief devices. • Otherwise, when the pressure builds up, • the stopper could be "rocketed out" • or worse, the container could explode

6. Appropriate Containers Examples of acceptable cryogenic Dewars:

7. Inappropriate Containers • DO NOT use open, un-insulated or glass containers!

8. Liquid Nitrogen Tank • Located off loading dock at Science Lab Building • Key can be checked out at the Chemistry storeroom. • Only trained personnel should dispense Liquid Nitrogen. • Enter through South East gate. Valve Location

9. Liquid Nitrogen Tank • Do NOT touch any of the valves on the North side of the tank. • Do NOT enter through the North gate. DO NOT touch!

10. Protective Clothing • Always wear thermal protective clothing when handling refrigerated/cryogenic liquids or solids. • It is required you wear a full face shield over safety glasses, loose fitting gauntlet gloves, long sleeve shirts, full-length trousers without cuffs, and fully enclosed shoes.

11. Fill Hose • The liquid Nitrogen is released through this hose from the outside tank.

12. Fill Hose • The liquid Nitrogen is released through this hose from the large inside dewar.

13. Procedures:Step 1: Fill Hose • Insert and hold the fill hose into the appropriate container or into the funnel.

14. Procedures:Step 2: Open/Close Valve • Open valve slowly to begin filling. • Close valve tightly after filling. Valve Valve

15. Safe Handling • Always wear personal protective equipment while handling liquid nitrogen. • Always use Liquid Nitrogen in a well ventilated area. • Use a loose-fitting stopper or lid that allows venting of the boil off. • If using a funnel, use caution not to overfill the container.

16. Safe HandlingContinued • Only transport liquid nitrogen in the freight elevator with no other occupants present. • Only use containers that have been designed specifically for cryogenic liquids. • Do not store in a confined space. • Do not store at temperatures above 125°F.

17. References • DOT Emergency Response Guide • CAMEO Chemicals Datasheet • Praxair Liquid Nitrogen Safety Data Sheet