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Growth of Microorganisms

How do the little bugs grow?. Growth of Microorganisms. Outline. Vocabulary warning Physical Requirements Chemical Requirements Patterns of Growth Measurements of Growth. Vocabulary Warning. There is a lot of vocabulary in the next two chapters

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Growth of Microorganisms

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  1. How do the little bugs grow? Growth of Microorganisms

  2. Outline • Vocabulary warning • Physical Requirements • Chemical Requirements • Patterns of Growth • Measurements of Growth

  3. Vocabulary Warning • There is a lot of vocabulary in the next two chapters • Much of the vocabulary is very specific in meaning and descriptive • These terms are extremely relevant to clinical microbiology and are part of the professional lexicon of the field.

  4. Physical Parameters of Growth • Like all machines, microbes have operational conditions. • Though microbes dominate every niche on the planet, not all microbes are everywhere. • The physical conditions of growth are directly linked to physiology.

  5. Example of Physical Parameters = Psycrophile = Psycrotroph = Mesophile 10° C 100° C = Thermophile = Hyperthermophile 0° C 37° C

  6. Other types of physical conditions • Salt concentration • Halophile • pH • Basophile • Acidophile • Water Concentration • Xerophile

  7. Why do these boundaries exist? • Structural Integrity of Cell Components • Protein Denaturation • Chicken and Egg Problem • Natural Environment of Cells • The Story of Hansen’s Disease

  8. Chemical Requirements for Growth. • What elements do we need to form the major macromolecules? • Carbon • Nitrogen • Sulfur • Phosphorus

  9. What about Oxygen and Hydrogen? • Both can be gotten from water. • Oxygen is a special case • Aerobe – organism that requires oxygen (O2) • Anaerobe – organism that does not require oxygen (O2) • Modifying Language • Obligate • Facultative

  10. Carbon, Nitrogen, Sulfur, and Phosphorus • Heterotroph vs. Autotroph • Must be present in a usable form • Is this everything you need to make a cell?

  11. Other Components • Trace Elements (i.e. metals) • Vitamins • Unique Compounds

  12. Types of Media • Complex Media • Defined Media

  13. Can we culture everything? NO, many things remain unculturable in today’s micro lab and these are proving to be some of the most interesting organisms.

  14. Pattern of Growth = Lag Phase = Log Phase = Stationary Phase = Death Phase

  15. Caveats to Model • Small Initial Inoculum • Media can be utilized • Culture is not used to the media • Generation Time, how can you calculate it?

  16. Explain this data

  17. Measuring Growth • Direct • Indirect

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