1. C2C12 myocytes Skeletal muscle 130 kDa OB-Rb b-actin 42 kDa Supplemental Figure 1. Western-blot analyses of leptin receptor (OB-R) in C2C12 myocytes and skeletal muscle using duplicate sample preparation incubated with a polyclonal antibody against the functional leptin receptor, OB-Rb. A single band of 130 kDa was detected in all experiments (n=8). Supplemental Fig.1