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Advanced Detection of Shigella Using Type III Needle scFv in Functional Assay

This study explores the efficacy of the Type III Needle scFv for the detection of Shigella in a functional assay. DH10B cells are incubated with his-tagged espA, followed by washing, lysis, and blotting on nitrocellulose. The detection is achieved through anti-his antibody staining. Negative controls are included for result validation. The analysis focuses on Displayers and their interactions with various proteins including ehaB, VtaA11, OprF, eCPX, yuaQ, azo1653, CPG_L2, espP(beta), and AIDA-1, illustrating the potential of these probes in probiotic applications.

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Advanced Detection of Shigella Using Type III Needle scFv in Functional Assay

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  1. Display-O-Matic Think Outside the Cell

  2. Display-O-matic Passenger: Type III Needle scFv • TypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex • Functional Assay: • Incubate DH10B cells with his-tagged espA • Wash and lyse cells • Dry lysate on nitrocellulose, block, and stain with anti-his antibody • Negative controls: • Results: • Displayers only: • Displayers + Needle scFv : 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 9 • Dot blot of …legend • ehaB, VtaA11, OprF, eCPX, yuaQ, azo1653, CPG_L2, espP(beta), AIDA-1

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