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Antimicrobial Activity of Herbal Plants

Antimicrobial Activity of Herbal Plants. By Sanjay Singh. http://powerpointpresentationon.blogspot.com. What are Herbal Plants ?. A plant whose stem does not produce woody, persistent tissue and generally dies back at the end of each growing season.

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Antimicrobial Activity of Herbal Plants

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  1. Antimicrobial Activity of Herbal Plants By Sanjay Singh http://powerpointpresentationon.blogspot.com

  2. What are Herbal Plants ? A plant whose stem does not produce woody, persistent tissue and generally dies back at the end of each growing season. Any of various often aromatic plants used especially in medicine or as seasoning. Example - Marijuana

  3. Materials and Methods Plants used for analysis D. eleta (Fabaceae), E.axillare, M. tridenta, M. cerviana and S. incanum (Solanaceae) Preparation of Herbal Plant Extract • 30 g of crushed plant powder mixed with 300 ml of methanol , chloroform and hexanol separately. • Keep it for 24 hrs. • Now filter the extract with filter paper.

  4. Preliminary Phytochemical Analysis There are several tecniques to identify phytochemicals in organic solvents of plant air-dried ext. • TLC • ultra violet • infrared spectroscopy • nuclear magnetic resonance • HPLC

  5. Test for alkaloids Add few drops of dil. Hcl to 2ml extract and then add 1ml of Dragendorff reagent ( a sol. of potassium bismuth iodide) if orange ppt. turns into red precipitate…..confirms presence of alkaloids. Test for tannins add few drops of lead acetate Pb(C2H3O2)2 to 2ml of each ext. formation of white ppt. confirms tannins.

  6. Test for Steroids and Triterpenoids Add 10ml of chloroform to 2ml of each ext. then add 1ml of acetic anhydride C4H6O3, then add 2ml of conc. sulphuric acid H2S04, if Blue- green colour appear shows that steroids is present and if red or pink colour at the junction appears it indicates presence of triterpenoids. Test for Cardiac Glycosides Add few drops of glacial acetic acid and ferric chloride, then add 3-4 drops of conc. H2SO4, blue green colour confirms presence of glycosides.

  7. Herbal drugs are best suited for: • PRIMARY HEALTH CARE • INFECTIOUS DISEASES • AIDS and other viral infections • Opportunistic infections • MDR infections (e.g. T.B., Malaria) • DEGENERATIVE & GERONTOLOGICAL CONDITIONS • Osteoporosis • Chronic arthritis like osteoarthritis and rheumatoid arthritis • Neurological like Alzheimer, Parkinsonism • Anti-aging • Metabolic disorders • Diabetes • Dyslipidemias • Other conditions • Microcirculatory disorders • Liver diseases • Immunostimulants • Anti-cancer • Drugs affecting male libido

  8. Standardization of Herbal drugsRaw Drugs • Correct taxonomic identification & authentication • Study on the medicinal part: root, stem, bark, leaves, flowers, fruits,nuts, gum, resins etc. • Collection details: Location, stage & development/ growth of the plants, time, pre-processing storage etc. • Organoleptic examination of raw drug: • Evaluation by means of sensory organs: touch, odour taste • Microscopic & molecular examination • Chemical composition (TLC, GLC, HPLC, DNA fingerprinting) • Biological activity of the whole plant

  9. MEDICINAL PRPERTIES OF IMPORTANT HERBS

  10. Results Among the 5 bacterial strains tested for antinacterial activity, B. subtilis was most susceptible inhibition zone ranging from 8.66 0.57 to 12 1 mm and P. aeruginosa was least susceptible. The methanol ext. of M. tridentata have strong antibacterial activity against gram +ive strains B. subtilis and S. aureus with inhibition zones of 11.33 0.57 to 12 0

  11. THANK YOU

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